| HPLC-ELSD测定三七药材及其制剂中三七皂苷R1、人参皂苷Rg1及Rb1的含量 |
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| 引用本文： | 沙东旭,张满来.HPLC-ELSD测定三七药材及其制剂中三七皂苷R1、人参皂苷Rg1及Rb1的含量[J].中国中药杂志,2005,30(2):112-115. |
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| 作者姓名： | 沙东旭 张满来 |
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| 作者单位： | 辽宁省药品检验所,中药室,辽宁,沈阳,110023 |
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| 摘 要： | 目的 :用HPLC-ELSD测定三七药材及其制剂血塞通注射液中三七皂苷R1、人参皂苷Rg1及Rb1的含量。方法 :色谱柱填料为十八烷基健合硅胶 ,流动相为乙腈 水 ,梯度洗脱 ;漂移管温度 10 5℃ ,载气流速 2.9L·min-1。结果 :三七皂苷R1、人参皂苷Rg1及Rb1分别在 0.4 5 0 6～ 2.2 5 30 ,1.4 772～ 7 386 0 ,1 2 0 6. 6～ 6 .0 330 μg呈良好线性关系 ;药材中 3种成分的平均回收率分别为 97 1% (RSD 1.9% ) ,96 8% (RSD 2.0 % ) ,97 0 % (RSD 2.2 % ) ;血塞通注射液中分别为 98.7% (RSD 1.9% ) ,98.5 % (RSD 1.8% ) ,98.1% (RSD 1.4 % )。结论 :该方法简便、准确、分离效果好 ,无干扰 ,可用于三七药材及血塞通注射液的质量评价。
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| 关 键 词： | 三七 血塞通注射液 三七皂苷R 人参皂苷Rg 人参皂苷Rb HPL-CELSD |
| 文章编号： | 1001-5302(2005)02-0112-04 |
| 收稿时间： | 2003/2/20 0:00:00 |
Determination of notoginsenoside R1, ginsenoside Rg1 and Rb1in Radix Notog inseng and its preparation by HPLC-ELSD |
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| SHA Dong-xu;ZHANG Man-lai.Determination of notoginsenoside R1, ginsenoside Rg1 and Rb1in Radix Notog inseng and its preparation by HPLC-ELSD[J].China Journal of Chinese Materia Medica,2005,30(2):112-115. |
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| Authors: | SHA Dong-xu;ZHANG Man-lai |
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| Affiliation: | Liaoning Institute for Drug Control, Shenyang 110023, China. |
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| Abstract: | OBJECTIVE: To establish the quantitative method of notoginsenoside R1, ginsenoside Rg1 and Rb1 in Radix Notoginseng and its preparation Xuesaitong injection by HPLC-ELSD. METHOD: The column was packed with 5 microm Diamonsil C18 stationary phase. The mobile phase consisted of acetonitrile-water, eluted in gradient mode. The temperature of drift tube was 105 degrees C and the nebulizer nitrogen flow rate was 2.9 L x min(-1). RESULT: The linear ranges of the three components were 0.456-2.25 microg, 1.47-7.38 microg and 1.20-6.03 microg respectively. The average recoveries of the three components in Radix Notoginseng were 97.1% (RSD 1.9%), 96.8% (RSD 2.0%), 97.0% (RSD 2.2%) respectively; in Xuesaitong Injection were 98.7% (RSD 1.9%), 98.5% (RSD 1.8%), 98.1% (RSD 1.4%) respectively. CONCLUSION: It was proved that the method was reliable, simple, and precise, that could be used for quality control. |
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| Keywords: | Radix Notoginseng Xuesaitong injection notoginsenoside R ginsenoside Rg ginsenoside Rb HPLC-ELSD |
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