| 蝎毒多肽提取物联合化疗抑制Lewis肺癌血管生成实验研究 |
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| 引用本文： | 孙晓佳,张月英,贾青,王兆朋,王朝霞,张维东.蝎毒多肽提取物联合化疗抑制Lewis肺癌血管生成实验研究[J].中国中药杂志,2011,36(12):1644-1649. |
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| 作者姓名： | 孙晓佳 张月英 贾青 王兆朋 王朝霞 张维东 |
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| 作者单位： | 山东省医学科学院基础医学研究所山东省现代医用药物与技术重点实验室,山东济南,250062 |
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| 基金项目： | 国家自然科学基金项目(30873408,81073102);山东省自然科学基金项目(Y2008C176);济南市科学技术发展计划项目(201004012) |
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| 摘 要： | 目的:观察蝎毒多肽提取物(polypeptide extract from scorpion venom,PESV)对Lewis肺癌荷瘤小鼠肿瘤血管生成的抑制作用并探讨可能的机制.方法:54只C57BL/6J小鼠皮下接种小鼠肺癌Lewis细胞,7 d后随机分为荷瘤对照组、化疗组和PESV组.以环磷酰胺对荷瘤小鼠进行化疗建立肿瘤化疗模型.按不同用药方法对3组小鼠进行干预,隔日测量肿瘤体积.各组每7 d处死6只小鼠,实验共进行28 d.以免疫组织化学方法检测各组肿瘤组织Ⅷ因子,α-SMA,D114及Notchl蛋白表达水平.以Ⅷ因子标记微血管,计算微血管密度(MVD),以α-SMA标记成熟有功能的血管,计算血管成熟度.以RT-PCR方法检测肿瘤组织D114,Notchl基因水平表达.结果:PESV抑瘤率为42.21%.PESV组肿瘤体积与化疗组存在明显差异(P＜0.05),与荷瘤对照组有显著差异(P＜0.01).PESV组Ⅷ因子表达水平显著低于化疗组(P＜0.05).化疗组肿瘤组织α-SMA表达低于荷瘤对照组(P＜0.01),PESV组显著低于化疗组(P＜0.01).化疗组Dll4及Notch1第28天表达低于荷瘤对照组(P＜0.05),PESV组肿瘤D114及Notch1第21天表达显著低于化疗组(P＜0.05).结论:PESV可对Lewis肿瘤血管生成起抑制作用,其机制可能与抑制肿瘤微环境中血管生成相关因子D114及Notch1有关.
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| 关 键 词： | 蝎毒液类 肺肿瘤 化疗 血管生成 |
| 收稿时间： | 2010/6/28 0:00:00 |
Effect of polypeptide extract from scorpion venom (PESV) with chemotherapy inhibited angiogenesis of lewis lung carcinomas |
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| SUN Xiaoji,ZHANG Yueying,JIA Qing,WANG Zhaopeng,WANG Zhaoxia and ZHANG Weidong.Effect of polypeptide extract from scorpion venom (PESV) with chemotherapy inhibited angiogenesis of lewis lung carcinomas[J].China Journal of Chinese Materia Medica,2011,36(12):1644-1649. |
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| Authors: | SUN Xiaoji ZHANG Yueying JIA Qing WANG Zhaopeng WANG Zhaoxia and ZHANG Weidong |
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| Affiliation: | Department of Experimengal Pathology and Pathophysiology, Institude of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Monder Medicine and Technology of Shandong Province, Jinan 250062,China;Department of Experimengal Pathology and Pathophysiology, Institude of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Monder Medicine and Technology of Shandong Province, Jinan 250062,China;Department of Experimengal Pathology and Pathophysiology, Institude of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Monder Medicine and Technology of Shandong Province, Jinan 250062,China;Department of Experimengal Pathology and Pathophysiology, Institude of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Monder Medicine and Technology of Shandong Province, Jinan 250062,China;Department of Experimengal Pathology and Pathophysiology, Institude of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Monder Medicine and Technology of Shandong Province, Jinan 250062,China;Department of Experimengal Pathology and Pathophysiology, Institude of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Monder Medicine and Technology of Shandong Province, Jinan 250062,China |
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| Abstract: | |
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| Keywords: | scorpion venoms lung cancer chemotherapy angiogenesis |
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