| 丹参对血管紧张素Ⅱ诱导的肾小球系膜细胞表达TGF-β1,ROS和PAI-1的影响 |
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| 引用本文： | 袁军,鲁艳芳 陈陶后,曾祥法.丹参对血管紧张素Ⅱ诱导的肾小球系膜细胞表达TGF-β1,ROS和PAI-1的影响[J].中国中药杂志,2007,32(22):2400-2404. |
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| 作者姓名： | 袁军 鲁艳芳 陈陶后 曾祥法 |
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| 作者单位： | 湖北中医学院 湖北武汉430061(袁军，鲁艳芳，陈陶后)，湖北中医学院中药教研室 湖北武汉430061(曾祥法) |
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| 摘 要： | 目的：探讨丹参对血管紧张素Ⅱ致肾小球硬化的干预作用。方法：采用血管紧张素Ⅱ刺激系膜细胞增殖,同时加入不同剂量的丹参注射液分别对系膜细胞作用24 h。观察系膜细胞纤溶酶原激活剂抑制物-1(plasminogen activator inhibitor-1,PAI-1)mRNA、培养上清液中的PAI-1蛋白和转化生长因子β1(transforming growth factorβ1,TGF-β1)的含量,以及细胞内活性氧(reactive oxygen species,ROS)水平的变化。结果：丹参明显抑制住了由血管紧张素Ⅱ刺激大鼠肾脏系膜细胞而产生的PAI-1 mRNA以及蛋白表达的升高,且其抑制作用的强弱与丹参注射液的剂量密切相关。丹参还明显下调了因血管紧张素Ⅱ刺激后TGF-β1的表达增加以及细胞内ROS水平的升高。结论：丹参下调血管紧张素Ⅱ诱导的系膜细胞PAI-1表达的增加,以及抑制TGF-β1的分泌增加与细胞内ROS水平的增高,从而发挥抗肾小球硬化的作用。
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| 关 键 词： | 丹参 纤溶酶原激活剂抑制物 转化生长因子β 细胞内活性氧 |
| 文章编号： | 1001-5302（2007）22-2400-05 |
| 收稿时间： | 2006-11-20 |
| 修稿时间： | 2006年11月20 |
Effects of Salvia miltiorrhiza on TGF-beta1, ROS and PAI-1 induced by angiotensin II in renal mesangial cells] |
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| YUAN Jun; LU Yan-fang; CHEN Tao-hou; ZENG Xiang-fa.Effects of Salvia miltiorrhiza on TGF-beta1, ROS and PAI-1 induced by angiotensin II in renal mesangial cells][J].China Journal of Chinese Materia Medica,2007,32(22):2400-2404. |
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| Authors: | YUAN Jun; LU Yan-fang; CHEN Tao-hou; ZENG Xiang-fa |
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| Affiliation: | 1. Pediatric Office Hubei College of Traditional Chinese Medicine, Wuhan 430061, China; 2. Chinese Medicine Teaching and Research, Hubei College of Traditional Chinese Medicine, Wuhan 430061, China |
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| Abstract: | OBJECTIVE: To investigate the effect of Salvia miltiorrhiza on glomerulosclerosis induced by Ang II. METHOD: Rat mesangial cells were exposed to 100 nmol L(-1) Ang II. Meanwhile, we added S. miltiorrhiza injection of different concentrations to Mcs. PAI-1 mRNA and protein, TGF-beta1 in serum free MEM medium, and the level of cellular reactive oxygen species (ROS) were measured. RESULT: S. miltiorrhiza notably attenuated Ang II induced expression of PAI-1 in a concentration-dependent manner. Meanwhile, S. miltiorrhiza suppressed the production of TGF-beta1 and cellular ROS in mesangial cells. CONCLUSION: S. miltiorrhiza can alleviate glomerular sclerosis. The renoprotective effects of S. miltiorrhiza may be due to its ability to decrease Ang II -induced PAI-1 and TGF-beta1 secretion and cellular ROS level. |
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