| 铁皮石斛花粉活力与种质创制研究 |
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| 引用本文： | 朱波,苑鹤,俞巧仙,斯金平.铁皮石斛花粉活力与种质创制研究[J].中国中药杂志,2011,36(6):755-757. |
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| 作者姓名： | 朱波 苑鹤 俞巧仙 斯金平 |
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| 作者单位： | 1. 浙江农林大学亚热带森林培育国家重点实验室培育基地天然药物研究开发中心,浙江,临安,311300
2. 浙江森宇药业有限公司,浙江,义乌,322000 |
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| 基金项目： | 浙江省重大科技专项(2009C12059) |
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| 摘 要： | 目的:为铁皮石解杂交育种与野生资源保护提供基础.方法:采用过氧化物酶法测定不同花期与不同贮藏条件的花粉活力;在温室大概设施栽培条件下开展杂交、自交、自由授粉,野外昆虫辅助授粉.结果:铁皮石斛有活力花粉比例花蕾期为29.4%,开花当天为70.6%,开花后1周为31.9%,直至花谢花粉仍有活力;离体干燥条件下4℃贮藏20 d后有活力花粉比例仍有21.2%;人工授粉杂交结实率达82.6%,自交结实率7.3%;野外昆虫辅助授粉与温室大棚设施栽培条件下自由授粉结实率均为0.结论:铁皮石斛花粉在整个花期均有活力,石斛花构造的特殊性导致自由授粉与野外昆虫辅助授粉均结实困难,人工杂交授粉是铁皮石斛野生资源的保护与种质创制的有效途径.
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| 关 键 词： | 铁皮石斛 花粉活力 人工授粉 果实生长 |
| 收稿时间： | 2010/8/10 0:00:00 |
Pollen vigor and development of germplasm of Dendrobium officinale |
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| ZHU Bo,YUAN He,YU Qiaoxian and SI Jinping.Pollen vigor and development of germplasm of Dendrobium officinale[J].China Journal of Chinese Materia Medica,2011,36(6):755-757. |
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| Authors: | ZHU Bo YUAN He YU Qiaoxian and SI Jinping |
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| Affiliation: | Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Research and Development Center for Natural Medicine, Zhejiang Agricultural and Forestry University, Lin'an 311300, China;Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Research and Development Center for Natural Medicine, Zhejiang Agricultural and Forestry University, Lin'an 311300, China;Zhejiang Senyu Pharmaceutical Co., Ltd., Yiwu 322300, China;Nurturing Station for the State Key Laboratory of Subtropical Silviculture, Research and Development Center for Natural Medicine, Zhejiang Agricultural and Forestry University, Lin'an 311300, China |
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| Abstract: | Objective: To provide the theoretical basis and applied technology for breeding superior species of Dendrobium officinale.Method: The peroxidase solution was used to test the pollen vigor in different flowering time and storage conditions.Cross,self and opening pollination were conducted in the green house,the subsidiary pollination by insects was carried out outdoors.Result: The pollen of D.officinale was still in vigor when the flower faded.The pollen vigor was only 29.4% in the buds,70.6% in the bloom da... |
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| Keywords: | Dendrobium officinale pollen vigor artificial pollination fruit growth |
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