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人参毛状根生物合成熊果苷的研究
引用本文:赵明强,丁家宜,刘峻,胡波.人参毛状根生物合成熊果苷的研究[J].中国中药杂志,2001,26(12):819-822.
作者姓名:赵明强  丁家宜  刘峻  胡波
作者单位:中国药科大学生物技术研究室,
摘    要:目的 :研究人参毛状根生物合成熊果苷的基本条件。方法 :以熊果苷的含量、氢醌 (1,4-对苯二酚 )的转化率为指标 ,找出适于生物合成的培养条件、持续时间和底物浓度。结果 :培养 22d的人参毛状根更换含底物的B5培养基后 ,对浓度为 2mmol·L-1的氢醌持续转化 24h ,所合成的熊果苷占干重的 13.0% ,转化率达 89.0%。结论 :本文首次以人参毛状根为反应器 ,人工合成出人参属植物所不具有的熊果苷。

关 键 词:人参毛状根  氢醌  生物合成  生物转化  糖基化  熊果苷
文章编号:1000-5302(2001)12-0819-04
收稿时间:2001/5/10 0:00:00
修稿时间:2001年5月10日

Studies on the Arbutin Biosynthesis by Hairy Root of Panax ginseng C.A Mayer
ZHAO Ming qiang;DING Jia yi;LIU Jun;HU Bo.Studies on the Arbutin Biosynthesis by Hairy Root of Panax ginseng C.A Mayer[J].China Journal of Chinese Materia Medica,2001,26(12):819-822.
Authors:ZHAO Ming qiang;DING Jia yi;LIU Jun;HU Bo
Affiliation:Institute of Biotechlogy, China Pharmaceutical University, Nanjing 210038 Jiangsu, China.
Abstract:Objective: To study basic conditions for biosynthesis arbutin by the hairy root of Panax ginseng . Method:Based on the content of arbutin and the biotransformation rate of hydroquione, these conditions such as the culture stage, the lasting time and the concentration of hydroquione fiting for biosynthesis were found. Result: After cultured for 22 days, transferred in fresh B5 medium having 2 mmol·L-1 hydroquione ,the hairy root biotransformated hydroquione into arbutin in 24 hours, the bioconversion rate was 89.0% and the content of arbutin in dry root was 13.0% . Conclusion: Arbutin, was which never found in the genus of Panax ginseng , can be biosynthesised by the hairy root of Panax ginseng .
Keywords:hairy root of Panax ginseng  hydroquione  biosynthesis  biotransformation  glycosylation  arbutin
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