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| 广防己、粉防己的肝肾毒性及代谢组学比较研究 | |
| 引用本文： | 梁琦,倪诚,颜贤忠,谢鸣,张艳霞,张琪,杨美娟,彭双清,张宇忠.广防己、粉防己的肝肾毒性及代谢组学比较研究[J].中国中药杂志,2010,35(21):2882-2888. |
| 作者姓名： | 梁琦 倪诚 颜贤忠 谢鸣 张艳霞 张琪 杨美娟 彭双清 张宇忠 |
| 作者单位： | 1. 北京中医药大学国家重点方剂学学科,北京,100029 2. 军事医学科学院,仪器分析中心,北京100850 3. 军事医学科学院,仪器分析中心,北京,100850 |
| 基金项目： | 国家自然科学基金项目(30572299) |
| 摘 要： | 目的:比较广防己、粉防己血、尿代谢表型差异,结合常规毒理学指标探讨广防己、粉防己的毒性差异。方法:雄性SD大鼠70只随机抽取10只为空白对照组,其余60只分为正常对照组、广防己组、粉防己组,每组20只。广防己、粉防己组按8.1 g.kg-1.d-1灌胃给药,正常对照组灌服等体积蒸馏水。连续给药4周。取给药前、给药2周、给药4周3个时间点各组大鼠的血液和收集24 h尿液,分别测定血、尿1H-NMR谱,并做血生化检测;取肝、肾脏做组织病理学检查。结果:给药2周时,较之正常对照组,广防己、粉防己组BUN,广防己组AST均升高(P0.05);较之广防己组,粉防己组SCr升高(P0.05),广防己、粉防己组肾组织均出现明显病理改变;粉防己组肝组织出现病理改变。给药4周时,较之正常对照组,广防己、粉防己组BUN,ALT,AST,广防己组Scr均显著升高(P0.01),肝、肾组织病理改变加重;较之广防己组,粉防己组BUN,SCr,AST均升高(P0.05)。广防己、粉防己组大鼠尿液中主要变化代谢物有柠檬酸,2-酮戊二酸,牛磺酸,马尿酸盐,TMAO,肌酐;血液中主要变化代谢物有不饱和脂肪酸,3-羟基丁酸,丙酮,乙酰乙酸,肌酐,NAC,OAC。结论:广防己、粉防己均可引起肝、肾损害,且随给药时间延长损伤加重;粉防己的肝、肾毒性较广防己重。 |
| 关 键 词： | 广防己 粉防己 肾毒性 肝毒性 代谢组学 核磁共振 模式识别 |
| 收稿时间： | 2009/12/3 0:00:00 |
Comparative study on metabonomics and on liver and kidney toxicity of Aristolochia fangchi and Stephania tetrandra |
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| LIANG Qi,NI Cheng,YAN Xianzhong,XIE Ming,ZHANG Yanxi,ZHANG Qi,YANG Meijuan,PENG Shuangqing and ZHANG Yuzhong.Comparative study on metabonomics and on liver and kidney toxicity of Aristolochia fangchi and Stephania tetrandra[J].China Journal of Chinese Materia Medica,2010,35(21):2882-2888. | |
| Authors: | LIANG Qi NI Cheng YAN Xianzhong XIE Ming ZHANG Yanxi ZHANG Qi YANG Meijuan PENG Shuangqing and ZHANG Yuzhong |
| Affiliation: | Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China;Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China;National Center of Biomedical Analysis, Beijing 100850, China;Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China;Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China;National Center of Biomedical Analysis, Beijing 100850, China;Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China;National Center of Biomedical Analysis, Beijing 100850, China;Department of Formulaology, Beijing University of Chinese Medicine, Beijing 100029, China |
| Abstract: | |
| Keywords: | Aristolochia fangchi Stephania tetrandra nephrotoxicity hepatotoxicity metabonomics nuclear magnetic resonace(NMR) pattern recognition |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
