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复方丹参注射液对内皮祖细胞数量和功能的影响
引用本文:季亢挺,张怀勤,杨鹏麟,李海鹰,杨德业,黄晓燕.复方丹参注射液对内皮祖细胞数量和功能的影响[J].中国中药杂志,2006,31(3):246-249.
作者姓名:季亢挺  张怀勤  杨鹏麟  李海鹰  杨德业  黄晓燕
作者单位:1. 温州医学院,附属第二医院,浙江,温州,325000
2. 温州医学院,附属第一医院,浙江,温州,325000
摘    要:目的:观察复方丹参注射液对内皮祖细胞(endothelial progenitor cells,EPCs)数量和功能的影响。方法:采用密度梯度离心法分离人脐血单个核细胞,接种到包被有人纤维连接蛋白的培养皿,分为对照组(未加细胞因子)、VEGF组(加血管内皮生长因子10 ng.mL-1)、复方丹参50组、复方丹参10组及复方丹参2组(分别加入复方丹参注射液50,10,2μg.mL-1),培养3 d后,倒置显微镜下观察集落形成,采用流式细胞术测定各组EPCs数,采用黏附能力测定试验测定黏附能力。结果:VEGF组、复方丹参10组及复方丹参2组的EPCs数显著高于对照组(分别P<0.01,P<0.05,P<0.01),且复方丹参2组显著高于复方丹参10组及VEGF组(均P<0.01);复方丹参50组的EPCs数显著低于对照组(P<0.01)。复方丹参10组及复方丹参2组的集落数及黏附细胞数显著高于对照组,而复方丹参50组则显著低于对照组。结论:低剂量复方丹参显著提高培养EPCs数量和功能,高剂量复方丹参则反之。

关 键 词:复方丹参注射液  内皮祖细胞  细胞培养
文章编号:1000-5302(2006)03-0246-04
收稿时间:2005-07-10
修稿时间:2005-07-10

Effects of Compound Salvia injection on number and activity of endothelial progenitor cells
JI Kang-ting,;ZHANG Huai-qin,;YANG Peng-Lin,;LI Hai-ying,;YANG De-Ye,;HUANG Xiao-Yan.Effects of Compound Salvia injection on number and activity of endothelial progenitor cells[J].China Journal of Chinese Materia Medica,2006,31(3):246-249.
Authors:JI Kang-ting  ;ZHANG Huai-qin  ;YANG Peng-Lin  ;LI Hai-ying  ;YANG De-Ye  ;HUANG Xiao-Yan
Affiliation:Department of Cardiology, the Second Affiliated Hospital of Wenzhou Medical College, Wenzhou 325000, China. jktdtl@sohu.com
Abstract:OBJECTIVE: To investigate the effects of Compound Salvia injection (CSI) on the number and activity of endothelial progenitor cells (EPCs). METHOD: Mononuclear fraction of human umbilical cord blood was obtained by density gradient centrifugation and plated on fibronectin coated culture dishes. Cells were divided in to five groups: group control, group VEGF, group CSI 50, group CSI 10 and group CSI 2 (supplemented with none cytokine, VEGF 10 ng x mL(-1), CSI 50, 10, 2 microg x mL(-1), respectively). After six days in culture, cell clusters were viewed with an inverted microscope, fluorescence-activated cell sorting (FACS) analysis of PE-CD34 and FITC-VE-Cadherin was performed to detect number of EPCs, adhesion assay was performed by replating cells on fibronectin coated dishes, and then counting adherent cells. RESULT: Numbers of EPCs of group VEGF, group CSI 10 and group CSI 2 were significantly increased as compared with those of group control ( P < 0.01, P < 0.05, P < 0.01, respectively), and numbers of EPCs of group CSI 2 were more than those of group CSI 10 and group V (P < 0.01). Compared with group control, number of EPCs of group CSI 50 was significantly decreased (P < 0.01). Compared with group control, numbers of clusters and adhesive EPCs of group CSI 10 and group CSI 2 were significantly increased, while those of group CSI 50 were significantly decreased. CONCLUSION: Low concentration CSI can significantly promote EPCs augmentation and enhance its functional activity, while high concentration CSI significantly restrains it.
Keywords:Compound Salvia injection  endothelial progenitor cells  cell culture
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