首页 | 官方网站   微博 | 高级检索  
     

基于ITS2条形码鉴定柴胡与大叶柴胡
引用本文:于俊林,赵莎,任明波,钱齐妮,庞晓慧.基于ITS2条形码鉴定柴胡与大叶柴胡[J].中国中药杂志,2014,39(12):2160-2163.
作者姓名:于俊林  赵莎  任明波  钱齐妮  庞晓慧
作者单位:通化师范学院 吉林省长白山药用植物研究重点实验室, 吉林 通化 134002;通化师范学院 制药与食品科学学院, 吉林 通化 134002;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193;重庆市药物种植研究所, 重庆 408435;重庆市药物种植研究所, 重庆 408435;中国医学科学院 北京协和医学院 药用植物研究所, 北京 100193
基金项目:科学技术部科技基础性工作专项(2014FY130400)
摘    要:为准确鉴定柴胡与大叶柴胡,该研究共48份柴胡、大叶柴胡药材,扩增ITS2 序列并进行测序,采用CodonCode Aligner软件进行序列拼接,用软件MEGA5.0分析比对确定不同的单倍型,并基于K2P模型进行遗传距离等分析。采用相似性搜索法、最近距离法、构建邻接树(NJ树)法对柴胡与大叶柴胡进行鉴定。结果显示柴胡与大叶柴胡种间最小K2P距离为0.049,远远大于柴胡种内最大K2P距离0.013。NJ树显示大叶柴胡独自聚为一支,可明显与柴胡区分。因此应用ITS2条形码可以准确鉴定柴胡及大叶柴胡。

关 键 词:大叶柴胡  柴胡  ITS2  DNA条形码
收稿时间:2014/3/14 0:00:00

Identification of Bupleurum chinense and B. longiradiatum based on ITS2 barcode
YU Jun-lin,ZHAO Sh,REN Ming-bo,QIAN Qi-ni and PANG Xiao-hui.Identification of Bupleurum chinense and B. longiradiatum based on ITS2 barcode[J].China Journal of Chinese Materia Medica,2014,39(12):2160-2163.
Authors:YU Jun-lin  ZHAO Sh  REN Ming-bo  QIAN Qi-ni and PANG Xiao-hui
Affiliation:Jilin Key Laboratory of Changbai Mountain Medicinal Plants Research, Tonghua Normal University, Tonghua 134002, China;Department of Pharmacy and Food Engineering, Tonghua Normal University, Tonghua 134002, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China;Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435, China;Chongqing Institute of Medicinal Plant Cultivation, Chongqing 408435, China;Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences & Peking Union Medical College, Beijing 100193, China
Abstract:In this study, ITS2 barcode was used to identify Bupleurum chinense and B. longiradiatum. The ITS2 regions of 48 samples were amplified and sequenced. The sequences obtained above were aligned and the K2P distances were calculated. We used three methods, BLAST1, nearest distance and phylogenetic tree (NJ-tree), to test the identification ability. The results showed that the maximum intraspecific genetic distance of B. chinense was 0.013, and the minimum interspecific genetic distance between B. chinense and B. longiradiatum was 0.049. The NJ-tree can easily identify B. chinense and B. longiradiatum. Therefore, the ITS2 barcode is suitable to identify B. chinense and B. longiradiatum.
Keywords:Bupleurum longiradiatum  B  chinense  ITS2  DNA barcoding
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号