| 基于matK基因的几种重要重楼属植物遗传关系分析 |
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| 引用本文： | 马剑,李迪强,张于光,薛达元.基于matK基因的几种重要重楼属植物遗传关系分析[J].中国中药杂志,2010,35(1):18-21. |
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| 作者姓名： | 马剑 李迪强 张于光 薛达元 |
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| 作者单位： | 1. 中央民族大学,生命与环境科学学院,北京,100081
2. 中国林业科学研究院,森林生态环境与保护研究所国家林业局,森林生态环境重点实验室,北京,100091 |
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| 基金项目： | 国家"十一五"科技支撑计划项目(2006BAD03A1202);国家科技基础条件平台建设项目(2005DKA21404) |
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| 摘 要： | 目的:利用叶绿体matK基因序列探讨重楼属植物的10个样品系统发育及分子鉴定,为重楼的保护和合理利用提基本供理论依据。方法:从分布于湖南省、云南省和吉林省的10个重要的重楼类群样品中提取总DNA,经扩增纯化后,利用PCR产物直接测序。结果:测定了10个重要的重楼类群样品的matK基因序列,序列长度为1 039 bp,比较了该基因的差异,构建了基于matK基因序列的遗传进化树。结论:这10个重楼属植物样品可以划分为4个组群,matK基因序列的结果不支持长药隔重楼、滇重楼、短梗重楼、七叶一枝花成为独立的变种,建议作为多叶重楼的变型处理。
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| 关 键 词： | 重楼属 matK基因 遗传关系 |
| 收稿时间： | 2009/4/14 0:00:00 |
Phylogenetic analyses of some important Paris species basedon sequences of matK gene |
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| MA Jian,LI Diqiang,ZHANG Yuguang and XUE Dayuan.Phylogenetic analyses of some important Paris species basedon sequences of matK gene[J].China Journal of Chinese Materia Medica,2010,35(1):18-21. |
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| Authors: | MA Jian LI Diqiang ZHANG Yuguang and XUE Dayuan |
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| Affiliation: | College of Life and Environmental Sciences, Minzu University of China, Beijing 100081;Institute of Forest Ecology, Environment and Protection, and Key Laboratory of Forest Ecology and Environment of StateForestry Administration, Chinese Academy of Forestry, Beijing 100091, China;Institute of Forest Ecology, Environment and Protection, and Key Laboratory of Forest Ecology and Environment of StateForestry Administration, Chinese Academy of Forestry, Beijing 100091, China;College of Life and Environmental Sciences, Minzu University of China, Beijing 100081 |
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| Abstract: | The matK genes of 10 samples in Paris from Hunan, Yunnan and Jilin provinces were sequenced and compared. The phylogenetic tree was constructed based on the matK gene sequences and the ten pairs samples were divided into four groups. The results did not support the reality of four taxa named P. polyphylla var. pseudothibetica,P. polyphylla var. yunnanensis, P. polyphylla var. appendiculata and P. polyphylla var. chinenis.They are supposed to be treated as different forms of P. polyphylla var.polyphylla. |
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| Keywords: | Paris matK gene genetic evolution |
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