| X-5大孔树脂纯化新乌头碱和乌头总碱 |
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| 引用本文： | 李文兰 王艳萍,季宇彬,范玉奇,赵培.X-5大孔树脂纯化新乌头碱和乌头总碱[J].中国中药杂志,2007,32(5):396-400. |
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| 作者姓名： | 李文兰 王艳萍 季宇彬 范玉奇 赵培 |
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| 作者单位： | 哈尔滨商业大学,药物研究所,博士后工作站,黑龙江,哈尔滨,150076 |
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| 基金项目： | 黑龙江省哈尔滨市科学计划 |
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| 摘 要： | 目的：对影响X-5树脂分离纯化乌头总生物碱和新乌头碱的各种因素进行系统的研究,使分离工艺达到最优化。方法：以川乌总碱和新乌头碱的含量为指标,考察X-5型大孔树脂分离纯化乌头总生物碱和新乌头碱的最佳吸附及洗脱条件。结果：X-5树脂纯化乌头总生物碱和新乌头碱的最佳工艺为上样液浓度含生药1 g·mL-1,药液pH 12,吸附时间为6 h,7 BV pH 8的95％乙醇洗脱效果好。X-5树脂可重复使用5次。经X-5树脂处理乌头总碱提取率可达80%以上,终产品总生物碱纯度可达30%以上。结论：该方法能明显提高新乌头碱和乌头总碱的纯度。
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| 关 键 词： | 大孔树脂 乌头总碱 新乌头碱 纯化 |
| 文章编号： | 1001-5302（2007）05-0396-05 |
| 收稿时间： | 2006-07-15 |
| 修稿时间： | 2006-07-15 |
Study on separation and purification of total alkaloids and mesaconitine with X-5 macroporous resin |
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| LI Wen-lan; WANG Yan-ping; JI Yu-bin; FANG Yu-qi; ZHAO Pei.Study on separation and purification of total alkaloids and mesaconitine with X-5 macroporous resin[J].China Journal of Chinese Materia Medica,2007,32(5):396-400. |
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| Authors: | LI Wen-lan; WANG Yan-ping; JI Yu-bin; FANG Yu-qi; ZHAO Pei |
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| Affiliation: | Institute of Materia Medica, Harbin University, Commerce Postdoctoral Programme, Harbin 150076, China. lwldzd@163.com |
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| Abstract: | Objective: To study systematically the factors which affect separation and purification of the total alkaloids and mesaconitine with X-5 macroporous resin. Method: With the content of the total alkaloids and mesaconitine as parameters, the optimum condition of absorption and elution were studied in the process of the purification with X-5 macroporous resin. Result: The X-5 macroporous resin yielded the best separating efficiency when the concentration of the extracted solution was 1g raw material per 1mL, pH 12.0 , the absorptive time of 6 hour and the volume of 95% ethanol (7BV pH 8) as the eluant; X-5 macroporous resins was used five times in a reproducible way. The rate of extraction and content of the total alkaloid were 80% and 30% respectively after purification with X-5 macroprous resin. Conclusion: The method can increase the purity of mesaconitine and total alkaloids. |
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| Keywords: | macroporous resins the total alkaloids in Radix Aconiti mesaconitine purification |
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