| 白藜芦醇对缺氧诱导大鼠乳鼠心肌细胞损伤的保护作用 |
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| 引用本文： | 黄秀兰,周秋兰,古丽丽,刘丹妮,李志勇,刘庆山,朱丹.白藜芦醇对缺氧诱导大鼠乳鼠心肌细胞损伤的保护作用[J].中国中药杂志,2010,35(1):94-98. |
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| 作者姓名： | 黄秀兰 周秋兰 古丽丽 刘丹妮 李志勇 刘庆山 朱丹 |
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| 作者单位： | 中央民族大学,中国少数民族传统医学研究院,北京,100081 |
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| 基金项目： | 国家民族事务委员会项目(08 zy 13);中央民族大学"985工程"项目 |
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| 摘 要： | 目的:观察白藜芦醇(Res)对缺氧诱导大鼠乳鼠心肌细胞损伤的保护作用。方法:建立体外培养大鼠乳鼠心肌细胞缺氧模型,MTT法检测心肌细胞活力,免疫组织化学法检测心肌细胞缺氧诱导因子-1α(HIF-1α)表达水平,检测心肌细胞培养液中乳酸脱氢酶(LDH)水平和心肌细胞内谷胱甘肽过氧化物酶(GSH-Px)活性。结果:大鼠乳鼠心肌细胞在体外缺氧培养24 h后,HIF-1α表达水平明显升高;心肌细胞培养液中LDH活性从正常对照组的(93.07±15.84)U.L-1上升到(750.77±181.51)U.L-1(P0.01);心肌细胞内GSH-Px活性从正常对照组(46.96±8.36)U.mL-1下降为(27.13±4.76)U.mL-1(P0.01)。25,50,75μmol.L-1Res可剂量依赖性抑制缺氧诱导大鼠乳鼠心肌细胞内HIF-1α表达增加;心肌细胞培养液中LDH含量呈剂量依赖性下降,分别为(486.17±69.97),(189.43±32.07),(155.34±29.57)U.L-1(P0.05或P0.01);心肌细胞内GSH-Px活性呈剂量依赖性升高,分别为(33.55±6.34),(37.67±6.73),(41.44±7.91)U.mL-1(P0.05或P0.01)。结论:Res对缺氧诱导大鼠乳鼠心肌细胞损伤有良好保护作用。
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| 关 键 词： | 白藜芦醇 大鼠乳鼠心肌细胞 缺氧 缺氧诱导因子-1α |
| 收稿时间： | 2009/6/10 0:00:00 |
Protective effects of resveratrol on neonatal rat cardiomyocytelesion induced by hypoxia |
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| HUANG Xiulan,ZHOU Qiulan,GU Lili,LIU Danni,LI Zhiyong,LIU Qingshan and ZHU Dan.Protective effects of resveratrol on neonatal rat cardiomyocytelesion induced by hypoxia[J].China Journal of Chinese Materia Medica,2010,35(1):94-98. |
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| Authors: | HUANG Xiulan ZHOU Qiulan GU Lili LIU Danni LI Zhiyong LIU Qingshan and ZHU Dan |
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| Affiliation: | Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China;Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China;Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China;Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China;Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China;Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China;Institute of Chinese Minority Traditional Medicine Minzu University of China, Beijing 100081, China |
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| Abstract: | Objective: To study the change of endogenous metabolites of SD rats administrated of aqueous extract of Evodiae rutaecarpa. Method: Six SD rats had been successively administrated aqueous extract of E. rutaecarpa(0.385 7 g·kg-1) for 33 days. An agilent 1200 6410 triplequadrupole mass spectrometer was used for the analysis of endogenous metabolites in rat urine samples. These data was analyzed by the principal component analysis (PCA) and PLS-DA using the SIMCA-P 10.0 software. Result: The significant difference in metabolic profiles between the control group and the dosed group was well observed by PCA of the MS data. Conclusion: The E. rulaecarpa has changed the endogenous metabolites of SD rats. This work can provide the base for the further research on the interpretation of drug property of E. rulaecarpa. |
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| Keywords: | resveratrol neonatal rat cardiomyocyte hypoxia hypoxia-inducible factor-1 alpha |
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