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| 叶黄素对食管癌细胞凋亡的影响及其作用机制研究 | |
| 引用本文： | 裴迎新,衡正昌,段广才,张遵真,王明臣,胡婵丽,高冬玲.叶黄素对食管癌细胞凋亡的影响及其作用机制研究[J].中国中药杂志,2007,32(4):332-334. |
| 作者姓名： | 裴迎新 衡正昌 段广才 张遵真 王明臣 胡婵丽 高冬玲 |
| 作者单位： | 1. 四川大学,华西公共卫生学院,四川,成都,610041;郑州大学,公共卫生学院,河南,郑州,450052 2. 四川大学,华西公共卫生学院,四川,成都,610041 3. 郑州大学,公共卫生学院,河南,郑州,450052 4. 郑州大学,基础医学院,河南,郑州,450052 |
| 基金项目： | 河南省医学科技攻关项目 |
| 摘 要： | 目的：探讨叶黄素对食管癌细胞凋亡的影响及其作用机制。方法：EC9706细胞在RPMI培养液(含10%小牛血清)中进行常规传代培养。常规条件下培养24 ｈ后,用叶黄素处理,同时设溶剂对照组和5-氟尿嘧啶(5-Fu)阳性对照组。采用流式细胞术观察叶黄素对细胞凋亡的影响,并用免疫组织化学方法检测其对凋亡调节蛋白Bax 和Bcl-2表达的影响。结果：在实验条件下,100 μg·mL-1叶黄素处理细胞96 h可促进细胞凋亡。免疫组化分析结果表明,同样条件下,叶黄素可抑制抗凋亡蛋白Bcl-2 的表达并促进促凋亡蛋白Bax的表达。结论：叶黄素具有促进EC9706细胞凋亡的作用,此作用可能是通过调节凋亡相关蛋白Bcl-2和Bax而实现的。 |
| 关 键 词： | 叶黄素 食管癌细胞EC9706 凋亡 |
| 文章编号： | 1001-5302（2007）04-0332-04 |
| 收稿时间： | 2006-08-23 |
| 修稿时间： | 2006-08-23 |
Effects and mechanism of lutein on apoptosis of esophageal carcinoma EC9706 cells |
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| PEI Ying-xin;; HENG Zheng-chang; DUAN Guang-cai; ZHANG Zun-zhen; WANG Ming-chen; HU Chan-li; GAO Dong-ling.Effects and mechanism of lutein on apoptosis of esophageal carcinoma EC9706 cells[J].China Journal of Chinese Materia Medica,2007,32(4):332-334. | |
| Authors: | PEI Ying-xin;; HENG Zheng-chang; DUAN Guang-cai; ZHANG Zun-zhen; WANG Ming-chen; HU Chan-li; GAO Dong-ling |
| Affiliation: | Huaxi School of Public Health, Sichuan University, Chengdu 610041, China. |
| Abstract: | OBJECTIVE: To study the effects of lutein on apoptosis and its mechanism. METHOD: The cells of human esophageal carcinoma EC9706 were grown in RPMI medium containing 10% bovine serum and were treated with lutein at 100 microg x mL(-1) concentration. Flow cytometry was employed to investigate the effects of lutein on cell apoptosis of EC9706 cells. Histochemistry was performed to determine apoptosis-related protein expresion. RESULT: Flow cytometry analyses revealed that lutein increased EC9706 cell apoptosis ratio when treated with lutein 100 microg x mL(-1) at 96 h. Lutein decreased the expression of Bcl-2 protein and increased the expression of Bax protein in EC9706 cells. CONCLUSION: Lutein could inhibit mitosis and stimulate apoptosis of EC9706 cells. The apoptotic effect may result from the down-regulation of expression of Bcl-2 and up-regulation expression of Bax. |
| Keywords: | lutein EC9706 cells apoptosis |
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