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川芎内生真菌的分离与鉴定
引用本文:汪杨丽,严铸云,郭晓恒,宋杰,陈新,万德光.川芎内生真菌的分离与鉴定[J].中国中药杂志,2008,33(9):999-1001.
作者姓名:汪杨丽  严铸云  郭晓恒  宋杰  陈新  万德光
作者单位:成都中医药大学药学院中药材标准化实验室,四川,成都,610075
摘    要:目的:探讨川芎内生真菌类群与川芎品种和产地的关系。方法:采用平板分离法分离川芎的内生真菌,采用点植法对分离菌株进行分类鉴定。结果:从6个产地的川芎根茎样品共获得内生真菌50株,经形态观察分类鉴定为1纲、3目、4科、13属。结论:不同产地及不同品种川芎的内生真菌在数量、分布、种群及其组成存在差异,推测川芎内生真菌种群可能和川芎的特定生境有关。

关 键 词:川芎  内生真菌  分离鉴定
文章编号:1001-5302(2008)09-0999-03
收稿时间:2006/12/5 0:00:00
修稿时间:2006年12月5日

Isolation and identification of endophytic fungi from Ligusticum
WANG Yang-li;YAN Zhu-yun;GUO Xiao-heng;SONG Jie;CHEN Xin;WAN De-guang.Isolation and identification of endophytic fungi from Ligusticum[J].China Journal of Chinese Materia Medica,2008,33(9):999-1001.
Authors:WANG Yang-li;YAN Zhu-yun;GUO Xiao-heng;SONG Jie;CHEN Xin;WAN De-guang
Affiliation:Chinese Traditional Medicine Standerdization Lab, Chengdu University of traditional Chinese medicine, Chengdu 610075, China.
Abstract:Objective: To investigate the correlation between entophytic fungal community,habitations and varieties of Ligusticum. Method: The solidified potato dextrose agar(PDA) of plates was applied for the isolation of the endophytic fungi,and the identification was completed by spot-planting method.Result: Fifty strains of the entophytic fungi were isolated from the rhizome of L.chuanxiong collected from 6 habitations.They were morphologically identified as belonging to 13 genera,4 families,3 orders and 1 class.Conclusion: There were some differences at quantity,species and distributing of the entophytic fungi in different habitats and varieties of Ligusticum,which was suggested that entophytic fungal community is related with specific habitat.
Keywords:Ligusticum  endophytic fungi  isolation and identification
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