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高效液相色谱法测定不同产地葛根中葛根素、大豆苷及大豆苷元的含量
引用本文:金文姗,谈钰元,陈有根,王妍.高效液相色谱法测定不同产地葛根中葛根素、大豆苷及大豆苷元的含量[J].中国中药杂志,2003,28(1):49-51.
作者姓名:金文姗  谈钰元  陈有根  王妍
作者单位:北京裕科植物中间体开发有限公司,北京,100007
摘    要:目的 :建立同时测定葛根中葛根素、大豆苷及大豆苷元的含量测定方法。方法 :选用SYMMETRY C18色谱柱 (3.9mm× 15 0mm ,5 μm) ,甲醇 1%醋酸水溶液为流动相梯度洗脱 ,紫外检测波长为 2 5 0nm ,HPLC法。结果 :平均回收率分别为 10 1.7% ,10 0 .7% ,10 1.7% (n=3) ,RSD分别为 0 .43 % ,0 .82 % ,1.5 0 % (n=3)。结论 :本法简便 ,准确 ,快速 ;适用于含葛根素、大豆苷及大豆苷元的葛根药材及制剂的质量控制。

关 键 词:二极管阵列高效液相色谱法  梯度洗脱  葛根素  大豆苷  大豆苷元  野葛
文章编号:1001-5302(2003)01-0049-03
收稿时间:2002/12/10 0:00:00
修稿时间:2002年12月10

Determination of Puerarin, Daidzin and Daidzein in Root of Pueraria lobata of Different Origin by HPLC
JIN Wen-shan;TAN Yu-yuan;CHEN You-gen;WANG Yan.Determination of Puerarin, Daidzin and Daidzein in Root of Pueraria lobata of Different Origin by HPLC[J].China Journal of Chinese Materia Medica,2003,28(1):49-51.
Authors:JIN Wen-shan;TAN Yu-yuan;CHEN You-gen;WANG Yan
Affiliation:Beijing Yuke Botanical Development CO., LTD., Beijing 100062, China. jinwenshan@yahoo.com.cn
Abstract:Objective: To develop an HPLC method to determine Puerarin, Daidzin and Daidzein in Pueraria lobata. Method: The separation was performed in a SYMMETRY C18 column with a mobile phase of methanol-1%HAC solution (gradient elution), the The detection wavelength being 250 nm. Result: The average recovery was respectively 101.7%,100.7% and 101.7%(n=3),RSD 0.43%, 0.82% and 1.50%(n=3) for Puerarin,Daidzin and Daidzein. Conclusion: This method is suitable for the determination of Puerarin,Daidzin and Daidzein in Pueraria lobata and its preparation.
Keywords:HPLC with DAD detection  gradient elution  Puerarin  Daidzin  Daidzein  Pueraria lobata
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