首页 | 官方网站   微博 | 高级检索  
     

单味关木通和龙胆泻肝汤肾毒性的比较研究
引用本文:张娜,谢鸣.单味关木通和龙胆泻肝汤肾毒性的比较研究[J].中国中药杂志,2007,32(7):619-622.
作者姓名:张娜  谢鸣
作者单位:北京中医药大学,国家重点方剂学学科,北京,100029
摘    要:目的:观察单味关木通、配伍关木通的龙胆泻肝汤给药后,大鼠肾功能、肾组织形态学及肾脏白介素-1β(IL-1β)、白介素-6(IL-6)水平变化。方法:大鼠随机分为单味关木通组、龙胆泻肝汤组、正常对照组3组,其中前两组分别按6,17.5 g·kg-1(其中含关木通6 g·kg-1)给以相应方药水提液灌胃,正常组给予等量饮用水,连续8周。测定大鼠血、尿生化指标及肾脏组织匀浆中IL-1β,IL-6水平,观察肾脏组织形态学改变。结果:2种水提液连续给大鼠灌胃8周,均可见明显的肾功能改变,肾脏组织匀浆中IL-1β,IL-6水平比正常组均明显升高,组织切片显示以炎症细胞浸润为主的肾小管间质病变;龙胆泻肝汤组的肾脏损伤较单味关木通组轻。结论:实验剂量的关木通与配伍关木通的龙胆泻肝汤均可引起肾功能损伤,肾脏损伤以炎症细胞浸润为主的肾小管间质病变和伴有高水平的IL-1β与IL-6为特征。推测关木通及其龙胆泻肝汤引起的肾毒性涉及免疫诱导的炎性损伤机制;复方龙胆泻肝汤肾毒性较低,可能与其减轻单味关木通引起的免疫诱导的炎性反应有关。

关 键 词:关木通  龙胆泻肝汤  肾毒性  炎症因子
文章编号:1001-5302(2007)07-0619-04
收稿时间:2006-04-17
修稿时间:2006-04-17

Nephrotoxicity of Aristolochia manshriensis and Longdan Xiegan decoction
ZHANG Na; XIE Ming.Nephrotoxicity of Aristolochia manshriensis and Longdan Xiegan decoction[J].China Journal of Chinese Materia Medica,2007,32(7):619-622.
Authors:ZHANG Na; XIE Ming
Affiliation:Department of formulaology, Beijing University of Traditional Chinese Medicine, Beijing 100029, China.
Abstract:OBJECTIVE: To observe the renal function, histology and the levels of interleukin-1beta (IL-1beta), interleukin-6 (IL-6) in rats administered with Aristolochi manshriensis (AM) and Longdan Xiegan decoction (LDXG) containing AM. METHOD: Divided SD rats into control, AM and LDXG groups (n = 10 per group) at random and administrated orally for 8 weeks with distilled water, AM (6 g x kg(-1) x d(-1)) and LDXG (17. 5 g x kg(-1) x d(-1), containing AM 6 g x kg(-1) x d(-1)), respectively. Measured renal functional parameters in serum, urine and IL-1beta, IL-6 in renal homogenate, and observe renal histologic lesions at the end of 8 weeks. RESULT: Two kinds of water abstract induced significantly renal injury in rats, IL-1beta, IL-6 levels in renal homogenate were significantly increased compared with controls, histological section showed inflammatory cell infiltration play important role in renal tubulointerstitium damage. LDXG caused less seriously damage than AM. CONCLUSION: AM and LDXG in experimental dosage can induce renal injury, inflammatory cell infiltration in renal tubulointerstitium and high levels of IL-1beta, IL-6 presumpt that the nephrotoxicity of AM and LDXG refered to the immune mechanism. LDXG has light nephrotoxicity due to its restraining the inflammatory reaction caused by AM.
Keywords:Aristolochia manshriensis  Longdan Xiegan decoction  nephrotoxicity  inflammatory factor
本文献已被 CNKI 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号