| 响应面法优化大孔树脂纯化昆仑雪菊色素的工艺 |
| |
| 引用本文： | 耿天华,汪涛,郭巧生,朱碧春.响应面法优化大孔树脂纯化昆仑雪菊色素的工艺[J].中国中药杂志,2016,41(2):220-225. |
| |
| 作者姓名： | 耿天华 汪涛 郭巧生 朱碧春 |
| |
| 作者单位： | 南京农业大学 中药材研究所, 江苏 南京 210095,南京农业大学 中药材研究所, 江苏 南京 210095,南京农业大学 中药材研究所, 江苏 南京 210095,南京农业大学 中药材研究所, 江苏 南京 210095 |
| |
| 摘 要： | 采用大孔树脂法纯化昆仑雪菊色素,应用响应面法(RSM)建立二次回归模型,优化昆仑雪菊色素纯化工艺。实验表明XDA-7树脂对昆仑雪菊色素的综合纯化效果最佳。昆仑雪菊色素吸附的最佳工艺为色素溶液浓度2.7 g·L-1,流速6 m L·min-1,p H 6,在此条件下的色素吸附率可达94.16%;最佳解吸条件为乙醇浓度64%,流速5 m L·min-1,洗脱用量4 BV,该条件下色素解吸率可达98.72%。
|
| 关 键 词： | 昆仑雪菊 色素 大孔树脂 响应面法 纯化 |
| 收稿时间： | 6/1/2015 12:00:00 AM |
Response surface optimization of purification process for pigment from Coreopsis tinctoria by macroporous resins |
| |
| GENG Tian-hu,WANG Tao,GUO Qiao-sheng and ZHU Bi-chun.Response surface optimization of purification process for pigment from Coreopsis tinctoria by macroporous resins[J].China Journal of Chinese Materia Medica,2016,41(2):220-225. |
| |
| Authors: | GENG Tian-hu WANG Tao GUO Qiao-sheng and ZHU Bi-chun |
| |
| Affiliation: | Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China,Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China,Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China and Institute of Chinese Medicinal Materials, Nanjing Agricultural University, Nanjing 210095, China |
| |
| Abstract: | To optimize the purification process of pigments from Coreopsis tinctoria with macroporous resins by establishing second regression model with response surface methodology. The experiment showed that XDA-7 resin had the best purification effect for pigments from C. tinctoria. The optimal absorption conditions for pigments from C. tinctoria were determined as follows: concentration of pigments solution 2.7 g·L-1, flow rate 6 mL·min-1, pH 6. Under these conditions, the absorption rate of pigments was up to 94.16%. Optimal desorption conditions were as follows: ethanol concentration 64%, flow rate 5 mL·min-1, elution dosage 4 BV. Under these conditions, pigment desorption rate was as high as 98.72%. |
| |
| Keywords: | Coreopsis tinctoria pigment the macroporous resin response surface methodology purify |
| 本文献已被 CNKI 等数据库收录！ |
|
