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一测多评法测定秦皮药材与饮片中香豆素类成分的含量
引用本文:冯伟红,王智民,张启伟,刘丽梅,王锦玉,杨菲.一测多评法测定秦皮药材与饮片中香豆素类成分的含量[J].中国中药杂志,2011,36(13):1782-1789.
作者姓名:冯伟红  王智民  张启伟  刘丽梅  王锦玉  杨菲
作者单位:1. 中国中医科学院中药研究所,北京,100700;中药质量控制技术国家工程实验室,北京,100700
2. 中国中医科学院基础理论研究所,北京,100700
基金项目:2007年中医药行业科技专业项目(200707009);国家"重大新药创制"科技重大专项(2009ZX09301-005,2009ZX09308-003)
摘    要:目的:建立适合于中药特点的利用一个对照品同步测定中药中多种香豆素类成分的分析方法,并进行方法学考察.方法:以中药秦皮为研究对象,以药材中典型成分秦皮甲索(aesculin)为参照物,建立该成分与秦皮乙素(asculetin)、秦皮苷(fraxin)和秦皮素(fraxetin)的相对校正因子.采用外标法测定秦皮甲素,用校正因子计算秦皮中其他3种香豆素类成分的含量;并将一测多评法的计算值与外标法实测值用相对误差进行评价,以验证一测多评法的准确性和适用性.结果:秦皮香豆素一测多评法的计算结果与外标法的实测值之间没有显著性差异,实验所得的校正因子可信.结论:一测多评的质量评价模式在中药秦皮的多指标质量评价中得到验证,可为后续《中国药典》的品种修订提供参考.

关 键 词:一测多评  HPLC  秦皮  香豆素  校正因子
收稿时间:2011/1/13 0:00:00

Quantitative method for simultaneous assay of four coumarins with one marker in Fraxini cortex
FENG Weihong,WANG Zhimin,ZHANG Qiwei,LIU Limei,WANG Jinyu and YANG Fei.Quantitative method for simultaneous assay of four coumarins with one marker in Fraxini cortex[J].China Journal of Chinese Materia Medica,2011,36(13):1782-1789.
Authors:FENG Weihong  WANG Zhimin  ZHANG Qiwei  LIU Limei  WANG Jinyu and YANG Fei
Affiliation:Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Basic Theory Research Institute of Chinese Medicine, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China;Institute of Chinese Materia Madica, China Academy of Chinese Medical Sciences, Beijing 100700, China;National Engineering Laboratory for Quality Control Technology of Chinese Herbal Medicines, Beijing 100700, China
Abstract:Objective : To establish a new quantitative method for simultaneous determination of multi-coumarins in Fraxini Cortex by using one chemical reference substance, and validate its feasibilities. Method: The new quality evaluation method, quantitative analysis of multi-components by singer-marker (QAMS), was established and validated with Fraxini Cortex. Four main coumarins were selected as analytes to evaluate the quality and their relative correlation factors (RCF) were determined by HPLC-DAD. Within the linear range, the values of RCF at 340 nm of aesculin to asculetin, fraxin and fraxetin were 1.771, 0.799, 1.409, respectively. And the contents of aesculin in samples of Fraxini Cortex were authentically determined by the external standard method, and the contents of the three other coumarins were calculated by their RCF. The contents of these four coumarins in all samples were also determined by the external standard method. Result: Within a certain range, the RCF had a good reproducibility (RSD 2.5%-3.9%). Significant differences were not observed between the quantitative results of two methods. Conclusion : It is feasible and suitable to evaluate the quality of Fraxini Cortex and its Yinpian by QAMS.
Keywords:quantitative analysis of multi-components by single-marker (QAMS)  HPLC  fraxini cortex  coumarins  relative correlation factor
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