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| 半边旗二萜化合物5F对HO-8910PM细胞中Nr1d1表达的影响 | |
| 引用本文： | 何太平,吴科锋,吕应年,龚先玲,George Gong Chen,梁念慈.半边旗二萜化合物5F对HO-8910PM细胞中Nr1d1表达的影响[J].中国中药杂志,2009,34(10):1268. |
| 作者姓名： | 何太平 吴科锋 吕应年 龚先玲 George Gong Chen 梁念慈 |
| 作者单位： | 1. 广东医学院检验学院,广东,湛江,524023 2. 广东天然药物研究与开发重点实验室,广东,湛江,524023 3. 香港中文大学,威尔士亲王医院外科系,香港沙田 |
| 基金项目： | 国家自然科学基金项目(3987099)；粤港科技合作项目(GHP/022/06) |
| 摘 要： | 目的:研究半边旗二萜化合物5F(5F from Pteris semipinnata L,PsL5F)对人高转移卵巢癌HO-8910PM细胞中核受体超家族成员1d1(nuclear receptor subfamily 1,group D,member 1,Nr1d1)表达的影响,探讨其抗肿瘤的可能作用机制.方法:培养HO-8910PM细胞,用0.1%DMSO,100 μmol~(-1) PsL5F分别处理HO-8910PM细胞24 h后,提取RNA和总蛋白,应用肿瘤基因芯片和荧光定量实时PCR检测PsL5F对Nr1d1 mRNA表达的影响;Western blot法分析PsL5F对Nr1d1蛋白表达水平的影响.结果:肿瘤基因芯片结果显示,100μmol·L~(-1) PsL5F处理组中Nr1d1 mRNA表达水平是对照组的26.17倍,荧光定量实时PCR结果与芯片结果吻合,PsL5F处理组中Nr1d1 mRNA表达水平是对照组的(35.34±1.07)倍(P<0.01),并且PsL5F处理组中Nr1d1蛋白表达水平是对照组的(7.71±0.43)倍(P<0.01).结论:PsL5F能明显上调HO-8910PM细胞中Nr1d1的表达,提示其与PsL5F抗肿瘤作用密切相关. |
| 关 键 词： | 半边旗 卵巢癌 Nr1d1 |
Effect of 5F from Pteris semipinnata on expression of Nr1d1 in HO-8910PM cell line |
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| HE Ta-Beng-,Tun-Ke-Feng-,Lv-Ying-Nian-,Gong-Xian-Ling-,George Gong Chen,Liang-Nian-Ci-.Effect of 5F from Pteris semipinnata on expression of Nr1d1 in HO-8910PM cell line[J].China Journal of Chinese Materia Medica,2009,34(10):1268. | |
| Authors: | HE Ta-Beng- Tun-Ke-Feng- Lv-Ying-Nian- Gong-Xian-Ling- George Gong Chen Liang-Nian-Ci- |
| Affiliation: | 1. School of Laboratory, Guangdong Medical College, Zhanjiang 524023, China; 2. Guangdong Key Laboratory for Research and Development of Natural Drugs, Zhanjiang 524023, China; 3. Department of Surgery, Prince of Wales Hospital, Chinese University of Hongkong, Hongkong, China |
| Abstract: | Objective: To investigate the effects of PsL5F (ent-11α-hydroxy-15-oxo-kaur-16-en-19-oic-acid, an extract from Pteris semipinnata L) on the expression of nuclear receptor subfamily 1, group D, member 1 (Nr1d1) in highly metastatic ovarian carcinoma HO-8910PM cells, and its mechanisms. Method: Microarray Chip was used to examine the level of Nr1d1 mRNA expression on HO-8910PM cells treated with PsL5F. Fluorescent quantitative real-time PCR assay and Western blot were performed to verify the effects of PsL5F on Nr1d1 mRNA and protein expression. Result: After 24 h treatment of 100 μmol·L-1 PsL5F, the mRNA and protein levels of Nr1d1 in HO-8910PM cells were 35.34±1.07 and 7.71±0.43 times compared to those of control group(P<0.01, P<0.01), respectively. Conclusion: PsL5F can up-regulate significantly the expression of Nr1d1 in HO-8910PM cells. Antitumor effects and its mechanisms of PsL5F in HO-8910PM cells may be involved in the up-regulation of Nr1d1 expression. |
| Keywords: | Pteris semipinnata ovarian carcinoma Nr1d1 |
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