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多产区川芎内生真菌菌群组成的PCR-DGGE分析
引用本文:王海,严铸云,何冬梅,王萌.多产区川芎内生真菌菌群组成的PCR-DGGE分析[J].中国中药杂志,2013,38(12):1893-1897.
作者姓名:王海  严铸云  何冬梅  王萌
作者单位:成都中医药大学 中药材标准化省部共建教育部重点实验室 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学 中药材标准化省部共建教育部重点实验室 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学 中药材标准化省部共建教育部重点实验室 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 四川 成都 611137;成都中医药大学 中药材标准化省部共建教育部重点实验室 中药资源系统研究与 开发利用省部共建国家重点实验室培育基地, 四川 成都 611137
基金项目:国家自然科学基金项目(81001610)
摘    要:研究采用PCR-DGGE和DNA测序技术,探究了四川5个不同产区间川芎内生真菌群的差异性.结果表明川芎内生真菌群落结构存在不同个体、不同产区间的差异,同产区不同个体川芎内生真菌的相似程度较高,不同产区间的相似程度较低;道地产区都江堰市石羊镇川芎的内生真菌群落结构较其他4个产区丰富,同时与其他产区相似度均很低,存在其他产区未见的特有种.综合分析认为在四川5产区中,都江堰市石羊镇的川芎内生真菌种群最为多样且群落结构相对固定,这可能是川芎道地性形成的重要原因.

关 键 词:川芎  内生真菌  PCR-DGGE
收稿时间:2012/10/24 0:00:00

Analysis of endophytic fungi community of Ligusticum chuanxiong using PCR-DGGE
WANG Hai,YAN Zhu-yun,HE Dong-mei and WANG Meng.Analysis of endophytic fungi community of Ligusticum chuanxiong using PCR-DGGE[J].China Journal of Chinese Materia Medica,2013,38(12):1893-1897.
Authors:WANG Hai  YAN Zhu-yun  HE Dong-mei and WANG Meng
Affiliation:Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine State, Key Labratorry Breeding Base of systematic Research Development and Utilization of Chinese Medicine Resourses, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine State, Key Labratorry Breeding Base of systematic Research Development and Utilization of Chinese Medicine Resourses, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine State, Key Labratorry Breeding Base of systematic Research Development and Utilization of Chinese Medicine Resourses, Chengdu 611137, China;Chengdu University of Traditional Chinese Medicine, The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine State, Key Labratorry Breeding Base of systematic Research Development and Utilization of Chinese Medicine Resourses, Chengdu 611137, China
Abstract:Objective: To research the diversity of endophytic fungal communities among Ligusticum chuanxiong growing at 5 areas in Sichuan province, and illuminate the developing mechanism of geoherbs from the microecological perspective. Method: The PCR-DGGE and DNA sequencing techniques were used to analyze the endophytic fungi community of L. chuanxiong. Result: The community of endophytic fungi present difference among different growing areas. Though minor difference were found among individuals at the same area, similarity among individuals from the same growing areas were higher significantly than those from different growing areas. Compared with the other 4 growing areas, L. chuanxiong from Shiyang town, Dujiangyan city had more abundant endophytic fungi and low similarity to others, and which probably had special types of fungi. Conclusion: The abundant and stable endophytic fungal community is an important factor for the development of geoherb L. chuanxiong at Shiyang town, Dujiangyan city.
Keywords:Chuanxiong  endophytic fungi  PCR-DGGE
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