首页 | 官方网站   微博 | 高级检索  
     

超高效液相色谱-飞行时间质谱法测定苦参素注射液中苦参碱和氧化苦参碱
引用本文:叶茂,谢国祥,赵爱华,张媛媛,贾伟.超高效液相色谱-飞行时间质谱法测定苦参素注射液中苦参碱和氧化苦参碱[J].中国中药杂志,2008,33(12):1398-1401.
作者姓名:叶茂  谢国祥  赵爱华  张媛媛  贾伟
作者单位:1. 上海交通大学药学院,上海,200240
2. 上海交通大学系统生物医学研究院,上海,200240
3. 上海交通大学药学院,上海,200240;上海交通大学系统生物医学研究院,上海,200240
基金项目:国家科技部国际合作项目
摘    要:目的:建立苦参素注射液中苦参碱和氧化苦参碱的含量测定方法。方法:色谱柱为Acquity UPLCBEH C18色谱柱(2.1 mm×100 mm,1.7 μm);以10 mmol·L-1醋酸胺水溶液(用氨水调节pH 8)-甲醇为流动相梯度洗脱;流速0.30 mL·min-1;柱温30 ℃;检测波长210 nm;进样量2 μL。结果:苦参碱和氧化苦参碱分别在0.084~3.36 μg和0.086~3.44 μg呈现良好的线性关系,平均加样回收率分别为103.2%,98.7 %;RSD分别为1.5%,1.2%。结论:本法简便、快速、准确,适用于该药品生产及临床应用中的质量监控。

关 键 词:超高效液相色谱  飞行时间质谱  苦参素注射液  苦参碱  氧化苦参碱
收稿时间:2007/12/19 0:00:00

Analysis of matrine and oxymatrine in kushensu injection byUPLC/Q-TOF-MS
YE Mao; XIE Guo-xiang; ZHAO Ai-hua; ZHANG Yuan-yuan; JIA Wei;.Analysis of matrine and oxymatrine in kushensu injection byUPLC/Q-TOF-MS[J].China Journal of Chinese Materia Medica,2008,33(12):1398-1401.
Authors:YE Mao; XIE Guo-xiang; ZHAO Ai-hua; ZHANG Yuan-yuan; JIA Wei;
Affiliation:School of Pharmacy, Shanghai Jiao Tong University, Shanghai 200240, China.
Abstract:OBJECTIVE: To develop a method for the determination of matrine and oxymatrine in Kushensu injection by ultra performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/Q-TOF-MS). METHOD: The analysis was performed on a Acquity UPLC BEH C18 column (2.1 mm x 100 mm, 1.7 microm) with the gradient elution of 10 mmol x L(-1) amine acetate (pH 8, adjusted by aqueous ammonia) and methanol. The flow rate was set as 0.30 mL x min(-1). The column temperature was maintained at 30 degrees C. The peaks were detected at 210 nm and the injection volume was 2 microL. RESULT: The calibration curves showed good linearity within the test ranges of 0.084-3.36 microg for matrine and 0.086-3.44 microg for oxymatrine, respectively. The mean recoveries were 103.2%, 98.7%, and the RSD were 1.5%, 1.2%, respectively. CONCLUSION: The established method is simple, rapid and sensitive, can be used for the quality control of matrine and oxymatrine in Kushensu injection through the manufacturing process and clinical implement.
Keywords:ultra high performance liquid chromatography (UPLC)  time-of-flight mass spectrometry (TOF-MS)  Kushensu Injection  matrine  oxymatrine
本文献已被 维普 万方数据 等数据库收录!
设为首页 | 免责声明 | 关于勤云 | 加入收藏

Copyright©北京勤云科技发展有限公司    京ICP备09084417号-23

京公网安备 11010802026262号