| 菊花-槐花总黄酮的制备工艺及质量控制 |
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| 引用本文： | 王如峰,雷帆,丁怡,邢东明,王倞,杜力军.菊花-槐花总黄酮的制备工艺及质量控制[J].中国中药杂志,2010,35(22):2980-2984. |
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| 作者姓名： | 王如峰 雷帆 丁怡 邢东明 王倞 杜力军 |
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| 作者单位： | 1. 清华大学生物科学学院,医学院系药物药理研究室,北京,100084;北京中医药大学中药学院,北京,100102
2. 清华大学生物科学学院,医学院系药物药理研究室,北京,100084 |
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| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09502-021,2009ZX09103-301) |
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| 摘 要： | 目的:优化菊花-槐花总黄酮的制备工艺,并建立总黄酮及指标成分的质量控制方法。方法:采用单因素试验和正交设计考察菊花-槐花总黄酮的制备和纯化工艺,分别用比色法和高效液相色谱法测定总成分和指标成分。结果:菊花-槐花总黄酮的制备工艺为处方药材用14倍量的70%乙醇回流提取3次,每次1 h;提取液浓缩至0.5 g.mL-1,以质量比1∶0.5的比例上样HPD 600大孔树脂柱,先用10倍树脂体积的蒸馏水洗去杂质,再用5倍树脂体积的80%乙醇解吸附;收集洗脱液,浓缩至干后,即得菊花-槐花总黄酮。经比色法测定工艺产品的总黄酮质量分数为64.98%~66.79%;经HPLC法测定其指标成分木犀草素-7-O-葡萄糖苷质量分数为5.87%~6.93%,芦丁质量分数为14.09%~16.62%。结论:含水乙醇提取结合大孔树脂柱纯化是制备菊花-槐花总黄酮的有效方法,比色法和高效液相色谱法可用于其质量控制。
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| 关 键 词： | 菊花 槐花 工艺 正交设计 质量控制 |
| 收稿时间： | 2010/2/26 0:00:00 |
Manufacturing process and quality control of total flavonoid from Chrysanthemum morifolium and Sophora japonica and its quality control |
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| WANG Rufeng,LEI Fan,DING Yi,XING Dongming,WANG Liang and DU Lijun.Manufacturing process and quality control of total flavonoid from Chrysanthemum morifolium and Sophora japonica and its quality control[J].China Journal of Chinese Materia Medica,2010,35(22):2980-2984. |
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| Authors: | WANG Rufeng LEI Fan DING Yi XING Dongming WANG Liang and DU Lijun |
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| Affiliation: | Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100102, China;Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China;Laboratory of Pharmaceutical Sciences, School of Life Sciences and School of Medicine, Tsinghua University, Beijing 100084, China |
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| Abstract: | |
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| Keywords: | Chrysanthemum morifolium Sophora japonica manufacturing process orthogonal experiment quality control |
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