| 西兰花中异硫氰酸盐诱导人肝癌细胞HepG-2凋亡作用及其机制的研究 |
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| 引用本文： | 季宇彬,池文杰,邹翔,郎朗,王志举.西兰花中异硫氰酸盐诱导人肝癌细胞HepG-2凋亡作用及其机制的研究[J].中国中药杂志,2007,32(7):612-615. |
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| 作者姓名： | 季宇彬 池文杰 邹翔 郎朗 王志举 |
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| 作者单位： | 1. 哈尔滨商业大学,生命科学与环境科学研究中心,黑龙江,哈尔滨,150076
2. 哈尔滨商业大学,药物研究所博士后科研工作站,黑龙江,哈尔滨,150076 |
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| 基金项目： | 国家自然科学基金;黑龙江省自然科学基金 |
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| 摘 要： | 目的：探讨西兰花中异硫氰酸盐(isothiocyanates,ITCS)诱导人肝癌细胞HepG-2凋亡过程中的作用及其可能机制。方法：不同浓度ITCS处理人肝癌细胞HepG-2,应用MTT法检测ITCS对HepG-2细胞增殖的影响；流式细胞仪检测活性氧、线粒体膜电位(Δψm)和细胞亚二倍体凋亡峰变化情况。结果：ITCS可明显抑制HepG-2细胞增殖；15,30,60,120,240 μg·mL-1的ITCS作用于HepG-2细胞24 h后,细胞内活性氧分别为(23.1±1.8)%,(53.3±3.3)%,(57.9±2.0)%,(79.9±3.4)%,(93.4±2.6)%；Δψm分别为(94.8±5.5)%,(91.8±5.4)%,(66.0±5.6)%,(65.5±6.6)%,(44.3±2.7)%；60,120,240 μg·mL-1的ITCS作用于HepG-2细胞48 h后可见细胞凋亡率分别为(16.6±2.8)%,(21.9±4.4)%,(70.2±5.3)%。结论：ITCS能够通过刺激HepG-2细胞内活性氧的产生,损伤线粒体,使其膜电位下降,引起HepG-2细胞凋亡。
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| 关 键 词： | 西兰花 异硫氰酸盐 细胞凋亡 活性氧 线粒体膜电位 |
| 文章编号： | 1001-5302（2007）07-0612-04 |
| 收稿时间： | 2006-09-27 |
| 修稿时间： | 2006-09-27 |
Study on apoptosis effect induced by isothiocyanates in Broccoli on HepG-2 cells and its mechanism |
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| JI Yu-bin; CHI Wen-jie; ZOU Xiang; LANG Lang; WANG Zhi-ju.Study on apoptosis effect induced by isothiocyanates in Broccoli on HepG-2 cells and its mechanism[J].China Journal of Chinese Materia Medica,2007,32(7):612-615. |
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| Authors: | JI Yu-bin; CHI Wen-jie; ZOU Xiang; LANG Lang; WANG Zhi-ju |
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| Affiliation: | Research Center on Life Sciences and Environmental Sciences, Harbin University of Commerce, Harbin 150076, China. jyb@hrbcu.edu.cn |
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| Abstract: | OBJECTIVE: To investigate the apoptosis effect of isothiocyanates (ITCS) on human liver cancer cells HepG-2, and its mechanism. METHOD: HepG-2 cells were treated with different concentrations of ITCS. MTT assay was used to evaluate the influence of ITCS on cell proliferation. Flow cytometry was used to test ROS levels, intracellular mitochondrial transmembrane potential (deltapsim) , and hypodiploid apoptosis peak in HepG-2 cells. RESULT: ITCS obviously inhibited proliferation of HepG-2 cells. When treated with 15, 30, 60, 120, 240 microg x mL(-1) of ITCS for 24 h, ROS levels were (23.1+/-1. 8)%, (53.3+/-3.3)%, (57.9+/-2.0)%, (79.9+/-3.4)%, (93.4+/-2. 6)% respectively; and deltapsim were (94.8+/-5.5)%, (91.8+/-5.4)%, (66.0+/-5.6)%, (65. 5+/-6.6)% and (44.3+/-2.7)% respectively; when treated with 60, 120, 240 microg x mL(-1) of ITCS for 48 h, cell apoptotic rates were (16.6+/-2.8)%, (21.9+/-4.4) % and (70.2+/-5.3) % respectively. CONCLUSION: ITCS generates ROS in gastric cancer HepG-2 cells, which causes mitochondrial membrane permeabilization and deltapsim decrease, therefore, leads to apoptosis of HepG-2 cells. |
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| Keywords: | Broccoli isothiocyanates apoptosis reactive oxygen species mitochondrial transmembrane potential |
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