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| 甘草Ri质粒转化及不同理化因子对甘草毛状根生长的影响 | |
| 引用本文： | 杨世海,刘晓峰,沈昕,郑俊华.甘草Ri质粒转化及不同理化因子对甘草毛状根生长的影响[J].中国中药杂志,2006,31(11):875-878. |
| 作者姓名： | 杨世海 刘晓峰 沈昕 郑俊华 |
| 作者单位： | 1. 吉林农业大学,中药材学院,吉林,长春,130118 2. 吉林亚泰(集团)股份有限公司,吉林,长春,130031 3. 北京林业大学,生物科学与技术学院,北京,100083 4. 北京大学药学院,北京,100083 |
| 摘 要： | 目的:研究甘草毛状根的诱导及外界因子对其生长的影响。方法:用发根农杆菌Agrobacterium rhizo-genesLBA9402和R1601感染甘草Glycyrrhiza uralensis外植体。结果:2种发根农杆菌均能诱导甘草产生毛状根,LBA9402比R1601表现出较强的对甘草的感染能力,下胚轴的转化率高于子叶。在WP培养基上毛状根生长最快。光照对毛状根生长有抑制作用。毛状根中5种黄酮类化合物的总量比愈伤组织中高1.5倍,其中甘草查耳酮含量是愈伤组织中的15.5倍。结论:本实验所建立的甘草毛状根培养系统,为研究甘草毛状根大量培养生产黄酮类化合物奠定了基础。 |
| 关 键 词： | 甘草 发根农杆菌 毛状根 黄酮类化合物 |
| 文章编号： | 1001-5302（2006）11-0875-04 |
| 收稿时间： | 7/1/2005 12:00:00 AM |
| 修稿时间： | 2005-07-01 |
Ri plasmid transformation of Glycyrrhiza uralensis and effects of some factors on growth of hairy roots |
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| YANG Shi-hai,;LIU Xiao-feng,;SHEN Xin,;ZHENG Jun-hua.Ri plasmid transformation of Glycyrrhiza uralensis and effects of some factors on growth of hairy roots[J].China Journal of Chinese Materia Medica,2006,31(11):875-878. | |
| Authors: | YANG Shi-hai ;LIU Xiao-feng ;SHEN Xin ;ZHENG Jun-hua |
| Affiliation: | 1. College of Chinese Medicinal Material, Jilin Agricultural University , Changchun 130118, China; 2. Jilin YaTai Group Co. Ltd. , Changchun 130031, China; 3. College of Biological Sdences and Technology, Belting Forestry University, Belting 100083, China; 4. School of Pharmaceutical Sciences, Peking University ,Beijing 100083 |
| Abstract: | Objective: To study the induction of hairy roots of Glycyrrhiza uralensis and effect of some physical as well as chemical factors on the growth of hairy roots. Method: Hypocotyls and cotyledons of G.uralensis were infected with Agrobacterium rhizogenes LBA9402 and A.rhizogenes R1601,respectively.Result: Hairy roots were induced by two strains of A.rhizogenes,and LBA9402 showed a stronger infective ability.The frequency of transformation of hypocotyls was higher than that of cotyledons.Among the different media tested,WP medium yielded the fastest growth rate of hairy roots.Light inhibited the growth of hairy roots.The total amout of 5 flavonoids in hairy roots was 1.5 times as much as that in the callus,among them licochalcone content was 15.5 times as that of callus.Conclusion: The in vitro culture system of the hairy roots established laid a foundation for mass production of flavonoids by hairy root culture. |
| Keywords: | Glycyrrhiza uralensis Agrobacterium rhizogenes hairy root flavonoids |
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