| 三七皂苷对阿霉素致心肌损伤保护作用的研究 |
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| 引用本文： | 师润,刘雳 霍杨,程翼宇.三七皂苷对阿霉素致心肌损伤保护作用的研究[J].中国中药杂志,2007,32(24):2632-2635. |
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| 作者姓名： | 师润 刘雳 霍杨 程翼宇 |
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| 作者单位： | 浙江大学药学院中药科学与工程学系,浙江,杭州,310058 |
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| 基金项目： | 国家重点基础研究发展计划(973计划) |
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| 摘 要： | 目的：研究三七皂苷对阿霉素所致心肌损伤的保护作用及其对阿霉素抗肿瘤活性的影响。方法：15 mg·kg-1腹腔注射阿霉素造成小鼠急性心肌损伤模型,观察不同剂量三七皂苷(25,50,100 mg·kg-1)干预对动物血清心肌酶谱指标及心肌组织中抗氧化酶活力的影响；采用大鼠心肌细胞H9C2,考察不同质量浓度三七皂苷(625～100 mg·L-1)减轻阿霉素心肌细胞毒性的作用；以MTT法测定三七皂苷对阿霉素抑制肿瘤细胞生长作用的影响。结果：阿霉素显著升高小鼠血清心肌酶指标,降低心肌组织抗氧化酶活力,不同剂量三七皂苷均能减轻心肌损伤、抑制心肌组织抗氧化酶活力的下降。阿霉素抑制离体培养心肌细胞的活力,三七皂苷能提高细胞存活率,但并不拮抗阿霉素对肿瘤细胞生长的抑制作用。结论：三七皂苷在体内和体外均显示减轻阿霉素心脏毒性的作用,同时并不影响后者抑制肿瘤细胞生长的作用。
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| 关 键 词： | 三七皂苷 阿霉素 心脏毒性 保护作用 |
| 文章编号： | 1001-5302（2007）24-2632-04 |
| 收稿时间： | 2007-06-10 |
| 修稿时间： | 2007年6月10日 |
Study on protective effects of Panax notoginseng saponins on doxorubicin-induced myocardial damage |
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| SHI Run; LIU Li; HUO Yang; CHENG Yi-yu.Study on protective effects of Panax notoginseng saponins on doxorubicin-induced myocardial damage[J].China Journal of Chinese Materia Medica,2007,32(24):2632-2635. |
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| Authors: | SHI Run; LIU Li; HUO Yang; CHENG Yi-yu |
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| Affiliation: | Zhejiang University, College of Pharmaceutical Sciences, Department of Sciences and Engineering of Traditional Chinese Medicines, Hangzhou 310058, China. |
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| Abstract: | OBJECTIVE: To study the effects of Panax notoginseng saponins (PNS) on acute doxorubicin-induced myocardial injury in mice and the anti-tumor efficiency of doxorubicin. METHOD: Mice were given a dose of 15 mg x kg(-1) doxorubicin ip alone or in combination with 25, 50, 100 mg x kg(-1) PNS ig, 5 days before doxorubicin administration and following 3 days. Cardiotoxic effects were measured by serum levels of dehydrogenase (LDH), creatine kinase (CK) and creatine kinase isoenzyme (CK-MB) and activities of antioxidant enzymes in heart tissue. In vitro experiments were performed using embryonic rat heart cell H9C2 to assess the protective effect of PNS (6.25-100 mg x L(-1)) against doxorubicin on cell viability. Anti-tumor efficiency of doxorubicin was evaluated by cytotoxic experiments using three cancer cell lines. RESULT: Pretreatment with PNS significantly lowered the levels of serum LDH, CK and CK-MB, and normalized myocardial superoxide dismutase, glutathione peroxidase and catalase activities. PNS also attenuated the inhibitory effect of doxorubicin on the viability of H9C2 cells, but did not compromise its inhibitory effect on proliferation of cancer cells. CONCLUSION: PNS was demonstrated to attenuate doxorubicin-induced myocardial damage without compromising its anti-tumor activity. |
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| Keywords: | Panax notoginseng saponins doxorubicin myocardial injury protection |
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