| 基于双向位点特异性PCR的金银花真伪鉴别方法研究 |
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| 引用本文： | 蒋超,张雅华,陈敏,袁媛,林淑芳,吴志刚.基于双向位点特异性PCR的金银花真伪鉴别方法研究[J].中国中药杂志,2012,37(24):3752-3757. |
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| 作者姓名： | 蒋超 张雅华 陈敏 袁媛 林淑芳 吴志刚 |
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| 作者单位： | 中国中医科学院中药研究所,北京,100700 |
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| 基金项目： | 国家自然科学基金项目(81001605);中药制药过程新技术国家重点实验室开放基金项目(SKL2010M0101) |
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| 摘 要： | 目的:筛选获得金银花真伪鉴别SNP位点并建立双向位点特异性PCR方法,用于鉴别金银花和其常见伪品以及两者的混杂品.方法:通过对GenBank收录的忍冬属植物叶绿体trnL-trnF序列进行对比分析,获得金银花真伪鉴别SNP位点；并依据该SNP位点设计特异性引物,对84份金银花基原植物及其市售饮片、伪品进行双向位点特异性PCR扩增,并根据真伪品的特异性条带进行金银花药材鉴别.结果:退火温度为61℃时,正品均出现468 bp的条带,红腺忍冬、华南忍冬、灰毡毛忍冬、黄褐毛忍冬、水忍冬、金银忍冬、郁香忍冬、新疆忍冬、繁果忍冬等9个伪品均出现324 bp的条带,在正品DNA中掺入5％以上伪品时同时出现正品和伪品条带.结论:双向位点特异性PCR可以鉴别金银花真伪品及两者的混杂样品.
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| 关 键 词： | 双向位点特异性PCR 金银花 分子鉴定 |
| 收稿时间： | 6/6/2012 12:00:00 AM |
Authentication of Lonicera japonica using bidirectional PCR amplification of specific alleles |
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| JIANG Chao,ZHANG Ya-hu,CHEN Min,YUAN Yuan,LIN Shu-fang and WU Zhi-gang.Authentication of Lonicera japonica using bidirectional PCR amplification of specific alleles[J].China Journal of Chinese Materia Medica,2012,37(24):3752-3757. |
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| Authors: | JIANG Chao ZHANG Ya-hu CHEN Min YUAN Yuan LIN Shu-fang and WU Zhi-gang |
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| Affiliation: | Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China;Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | |
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| Keywords: | bidirectional PCR amplification of specific alleles Lonicera japonica molecular authentication |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
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