| 巴戟天寡糖对皮质酮损伤的PC12细胞的保护作用 |
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| 引用本文： | 李云峰,杨明,赵毅民,罗质璞.巴戟天寡糖对皮质酮损伤的PC12细胞的保护作用[J].中国中药杂志,2000,25(9):551-554. |
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| 作者姓名： | 李云峰 杨明 赵毅民 罗质璞 |
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| 作者单位： | 军事医学科学院毒物药物研究所,北京 |
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| 摘 要： | 目的 :探讨巴戟天寡糖 (MW 97)抗抑郁作用的可能机制。方法与结果 :MW-97或NGF与皮质酮 2×10 -4 mol·L-1共孵PC12细胞 48h ,可防止皮质酮所致的PC12神经细胞损伤。进一步运用RT PCR方法研究表明 ,MW 97(10 0mg·kg-1)及去甲丙米嗪 (10mg·kg-1)慢性ip 21d ,可使大鼠前脑皮层NGF、脑源性神经营养因子(BDNF)及海马BDNFmRNA表达升高。结论 :MW 97抗抑郁作用机理可能与神经细胞营养因子表达升高 ,从而对皮质酮诱导的神经元损伤产生保护作用有关。
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| 关 键 词： | 巴戟天寡糖 皮质酮 PC12细胞 神经营养因子 |
| 收稿时间： | 1999/12/7 0:00:00 |
Protective Effect of Bajitian oligosaccharide on PC12 Cells Lesioned by Corticosterone |
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| LI Yun-feng;YANG Ming;ZHAO Yi-min;LUO Zhi-pu.Protective Effect of Bajitian oligosaccharide on PC12 Cells Lesioned by Corticosterone[J].China Journal of Chinese Materia Medica,2000,25(9):551-554. |
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| Authors: | LI Yun-feng;YANG Ming;ZHAO Yi-min;LUO Zhi-pu |
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| Affiliation: | Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences, Beijing 100850, China. |
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| Abstract: | objective:To study the possible mechanism of antidepressant effect of Bajitian oligosaccharide(MW-97).Methods and results:After incubating with the PC12 cells in the presence of corticosterone(2×10 -4mol·L-1)for 48h,it was found that MW-97 could protect PC12 cells from the lesion done by corticosterone in a concentration-dependent manner.Forthermore,using RT-PCR mediated cross-species partial cDNA cloning,it was found that MW-97 and desipramine(DIM)increased NGF,BDNF mRNA in the frontal cortex after chronic administration for 21 days,meanwhile,BDNF mRNA in hippocampus was also increased. Conclusion:The cytoprotective effect of MW-97 consists in the possible mechanism of its antidepressant action, while the increasing expression of neurotrophic factors (NGF,BDNF mRNA) may be contributes to the cytoprotective effect. |
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| Keywords: | Bajitian oligosaccharide corticosterone PC12 cells neurotrophic factors |
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