| 野葛毛状根的诱导及离体培养 |
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| 引用本文： | 刘传飞,李玲,施和平,潘瑞炽,于树宏,.野葛毛状根的诱导及离体培养[J].中国中药杂志,2000,25(9):525-527. |
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| 作者姓名： | 刘传飞 李玲 施和平 潘瑞炽 于树宏 |
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| 作者单位： | [1]江西农业大学农学院,江西南昌 [2]华南师范大学生物系,广东广州 |
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| 基金项目： | 广东省重点科技项目!资助 (S980 35 1)，江西自然科学基金!(S9940 1) |
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| 摘 要： | 目的； 野葛毛状根的诱导和无激素离体培养。 方法：用含超强毒性基因的发根农杆菌R1601与野葛离体叶片共培养。 结果：经感染的野葛叶片表面直接形成大量生长快、分枝多、负向地性的毛状根，毛状根离体培养具有抗卡那霉素特性和激素自给特征。 结论：建立了发根农杆菌诱导野葛毛状根的方法和野葛毛状根的离体培养系统。
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| 关 键 词： | 野葛 毛状根 离体培 |
| 收稿时间： | 1999/12/6 0:00:00 |
Haity Root Inducfion and in Vitro Culivation of Pueraria lobata Willa. Ohwi |
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| LIU Chuan-fel;Li Ling;SHI He-ping;PAN Rui-chi;YU shu-hong.Haity Root Inducfion and in Vitro Culivation of Pueraria lobata Willa. Ohwi[J].China Journal of Chinese Materia Medica,2000,25(9):525-527. |
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| Authors: | LIU Chuan-fel;Li Ling;SHI He-ping;PAN Rui-chi;YU shu-hong |
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| Abstract: | Objective: Tostua the hairy root induction and hormone-free in vitro liquid cultivation of Pueraria lobata (Willd) Ohwi. Method: Co-cultivation of super-virulent Agrobacterium rhizogenes R1601 with P. lobata leaves in uitro. Results: Hairy roots of rapid growth, high branches and plagiotropism developed vigorously on the surface of leaves, exhibiting rapid growth and resistance to kanamycin in hor-mone-free medium in vitro. Conclusion: A method of hairy root induction with A. rhizogenes as well as a system hairy root liquid cultivation in uitro for p.lobata have been established. |
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| Keywords: | pueraria lobata:hairy rooot cultivation in vitro |
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