| 药用肉苁蓉的遗传多样性RAPD分析 |
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| 引用本文： | 崔光红,陈敏,黄璐琦肖苏萍,李达.药用肉苁蓉的遗传多样性RAPD分析[J].中国中药杂志,2004,29(8):727-730. |
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| 作者姓名： | 崔光红 陈敏 黄璐琦肖苏萍 李达 |
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| 作者单位： | 中国中医研究院,中药研究所,北京,100700 |
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| 基金项目： | 科技部社会公益研究项目，科技部科技攻关项目 |
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| 摘 要： | 目的 :荒漠肉苁蓉Cistanche deserticola和管花肉苁蓉Cistanche tubulosa群体遗传多样性的研究。方法 :对荒漠肉苁蓉 2个野生居群和管花肉苁蓉 4个野生居群进行RAPD分析 ,利用POPGENE1.31版软件分析群体的遗传多样性。结果 :荒漠肉苁蓉平均多态位点百分率 47.37% ,2个居群多态位点分别为 39.4 7%和 35.53% ,平均Nei’s基因多样性为 0 .135 8,Shannon’s多态性信息指数为 0.2072 ,基因分化系数Gst=0.2546。管花肉苁蓉平均多态位点百分率 27.5 9% ,各居群多态位点百分率从 19.54 %到 25.29% ,其中安迪尔居群的最高 25.29% ,平均Nei’s基因多样性为 0.0823,Shannon’s多态性信息指数为 0.1258,基因分化系数Gst=0.1755。结论 :荒漠肉苁蓉和管花肉苁蓉在居群间均有一定的分化 ,荒漠肉苁蓉的遗传多样性明显高于管花肉苁蓉 ,并已明显分化成 2个生态型。
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| 关 键 词： | 荒漠肉苁蓉 管花肉苁蓉 RAPD 遗传多样性 |
| 文章编号： | 1001-5302(2004)08-0727-04 |
| 收稿时间： | 2004/3/11 0:00:00 |
Study on genetic diversity of Herba Cistanches by RAPD |
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| CUI Guang-hong;CHEN Min;HUANG Lu-qi;XIAO Su-Ping;LI Da.Study on genetic diversity of Herba Cistanches by RAPD[J].China Journal of Chinese Materia Medica,2004,29(8):727-730. |
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| Authors: | CUI Guang-hong;CHEN Min;HUANG Lu-qi;XIAO Su-Ping;LI Da |
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| Affiliation: | Institute of Chinese Materia Medica, Academy of Traditional Chinese Medicine, Beijing 100700, China. |
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| Abstract: | Objective: To determine the genetic diversity of Cistanche species. Method: Two populations of Cistanche deserticola and four populations of C. tubulosa were analyzed by random amplified polymorphic DNA (RAPD) markers. Result:A total of 76 and 87 loci were amplified using 10 random primers each other. The average percentage of polymorphic loci of C. deserticola was 47.37%. The PPL were 39.47% and 35.53% for two populations. Average Nei's gene diversity was 0.135 8, Shannon's genetic diversity was 0.207 2, and Gst was 0.254 6. The average PPL of C. tubulosa was 27.59%. It was 19.54% to 25.29% in different populations and Andi'er population had the highest. Average Nei's gene diversity was 0.082 3, and Shannon's genetic diversity was 0.125 8, Gst was 0.175 5. Conclusion: The diversity of Cistanche deserticola is higher than that of C. tubulosa, but both has differentiation among populations, C. deserticola has already separated itself into two different ecotypes. |
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| Keywords: | Cistanche deserticola C tubulosa RAPD genetic diversity |
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