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鹿茸中促大鼠成骨样细胞增殖活性组分的纯化与表征
引用本文:林冬云,黄晓南,柯李晶,陈晓超,叶秀云,霍玉书,饶平凡.鹿茸中促大鼠成骨样细胞增殖活性组分的纯化与表征[J].中国中药杂志,2005,30(11):851-855.
作者姓名:林冬云  黄晓南  柯李晶  陈晓超  叶秀云  霍玉书  饶平凡
作者单位:1. 福州大学,生物工程研究所,福建,福州,350002
2. 美国德州大学,胜安东尼奥健康科学中心,美国,德州,78229-3900
摘    要:目的:通过鹿血清白蛋白对大鼠成骨样细胞系UMR-106增殖活性和促胰岛素样生长因子Ⅰ(IGF-Ⅰ)分泌水平的研究,为阐明鹿茸强筋健骨的作用机理及对骨质疏松的防治提供研究线索。方法:运用SephacrylS-200HR凝胶色谱、POROS20QE阴离子交换色谱和TSKG3000SW高效液相色谱等分离手段,从冷冻干燥鹿茸粉粗提液中分离纯化得到鹿血清白蛋白。并用MTT法检测其对UMR-106细胞的增殖活性;用平衡饱和竞争放射免疫分析法(RIA)测定其促IGF-Ⅰ分泌水平。结果:经分离纯化得到的鹿血清白蛋白分子质量为56.3kDa,在蛋白含量为0.149μg·mL-1时,其促大鼠成骨样细胞增殖率为241.03%,IGF-Ⅰ分泌量为66.89ng·mL-1。结论:鹿茸中的鹿血清白蛋白在14.9ng·mL-1-14.90μg·mL-1具有显著的促大鼠成骨样细胞增殖和IGF-Ⅰ分泌等活性。

关 键 词:鹿茸  鹿血清白蛋白  分离纯化  UMR-106细胞  胰岛素样生长因子Ⅰ
文章编号:1001-5302(2005)11-0851-05
收稿时间:2004/8/31 0:00:00

Purification and characterization of the proliferation of rat osteoblast-like cells UMR-106 from Pilose Antler
LIN Dong-yun;HUANG Xiao-nan;KE Li-jing;CHEN Xiao-chao;YE Xiu-yun;HUO Yu-shu;RAO Ping-fan.Purification and characterization of the proliferation of rat osteoblast-like cells UMR-106 from Pilose Antler[J].China Journal of Chinese Materia Medica,2005,30(11):851-855.
Authors:LIN Dong-yun;HUANG Xiao-nan;KE Li-jing;CHEN Xiao-chao;YE Xiu-yun;HUO Yu-shu;RAO Ping-fan
Affiliation:Institute of Biotechnology, Fuzhou University, Fuzhou 350002, China.
Abstract:OBJECTIVE: The activity of deer serum albumin on proliferation of rat osteogenic-like cells UMR-106 and the IGF-I secretion were investigated in order to elucidate pilose antler's bone-strengthening mechanism. METHOD: Deer serum albumin was isolated from freeze-dry pilose antler powder extract. The methods were Sephacryl S-200HR gel filtration, POROS 20QE ion-exchange and TSK G3000SW chromatographies. The effect of deer serum albumin on proliferatio of UMR-106 cells was assaied by MTT, and the secretion of IGF-I of UMR-106 cells was assaied by RIA. RESULT: Deer serum albumin, with the molecular weight of 56.3 kDa, significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF-I secretion. When concentration of deer serum albumin reached 0.149 microg x mL(-1), UMR-106 cell proliferation rate was 241.03% and IGF-I secretion was 66.89 ng x mL(-1). CONCLUSION: The concentration of deer serum albumin, from 14.9 ng x mL(-1) to 14.90 microg x mL(-1), significantly increased the proliferation rate of the osteoblast-like UMR-106 cell and IGF- I secretion.
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