| 鸡血藤抗肿瘤活性部位SSCE指纹图谱的研究 |
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| 引用本文： | 王宏,刘艺娜,曾祖平,何薇.鸡血藤抗肿瘤活性部位SSCE指纹图谱的研究[J].中国中药杂志,2011,36(18):2525-2529. |
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| 作者姓名： | 王宏 刘艺娜 曾祖平 何薇 |
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| 作者单位： | 1. 首都医科大学附属北京中医医院北京市中医研究所,北京,100010
2. 首都医科大学附属北京中医医院,北京,100010 |
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| 基金项目： | 北京市科技项目(H010910190119) |
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| 摘 要： | 目的:建立鸡血藤黄酮类抗肿瘤活性部位( SSCE)的HPLC-DAD色谱指纹图谱,全面完整的反映了本部位内在的化学信息,并初步指认其中的主要化学成分.方法:采用HPLC-DAD检测,梯度洗脱的分析方法.Kromasil 100-5 PHENYL色谱柱(4.6 mm×250 mm,5μm)；流动相0.5％冰醋酸水溶液—甲醇；检测波长254 nm.结果:建立了10批鸡血藤药材中SSCE的HPLC-DAD色谱指纹图,获得了16个共有峰,并有10个色谱峰被指认.其中,l,3,4,5,8,9,10,12,13,16号分别被确定为原儿茶酸,对羟基苯甲酸,表儿茶素,葛根素,大豆苷元,甘草素,毛蕊异黄酮,染料木素,芒柄花素和樱黄素.结论:方法简便,精密度、重现性和稳定性良好,能有效控制中药鸡血藤黄酮类抗肿瘤活性部位( SSCE)的质量,为筛选鸡血藤抗肿瘤活性成分、进行药理研究提供质量保证.
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| 关 键 词： | HPLC 鸡血[藤 中药指纹图谱 活性部位 |
| 收稿时间： | 2010/11/26 0:00:00 |
Study on HPLC chromatographic fingerprint of anti-tumor active site SSCE of Caulis spatholobi |
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| WANG Hong,LIU Yin,ZENG Zuping and HE Wei.Study on HPLC chromatographic fingerprint of anti-tumor active site SSCE of Caulis spatholobi[J].China Journal of Chinese Materia Medica,2011,36(18):2525-2529. |
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| Authors: | WANG Hong LIU Yin ZENG Zuping and HE Wei |
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| Affiliation: | Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010,China;Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010, China;Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010,China;Beijing Institute of Traditional Chinese Medicine, Beijing Hospital of Traditional Chinese Medicine Affiliated to Capital Medical University, Beijing 100010,China |
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| Abstract: | |
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| Keywords: | HPLC Caulis Spatholobi Fingerprints active site |
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