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| 树豆菧类化合物longistylin A和longistylin C的检测 | |
| 引用本文： | 王达武,肖敏勋,李宜融,沈小玲,卢元媛,刘抗伦,李正红,胡英杰.树豆菧类化合物longistylin A和longistylin C的检测[J].中国中药杂志,2011,36(19):2680-2683. |
| 作者姓名： | 王达武 肖敏勋 李宜融 沈小玲 卢元媛 刘抗伦 李正红 胡英杰 |
| 作者单位： | 1. 广州中医药大学热带医学研究所药学研究室,广东广州,510405 2. 广州中医药大学热带医学研究所药学研究室,广东广州510405;国立中医药研究所中药标本馆,台湾台北 3. 中国林业科学研究院资源昆虫研究所,云南昆明,650224 |
| 基金项目： | 国家"重大新药创制"科技重大专项(2009ZX09103-436) |
| 摘 要： | 目的:建立以药物活性和专属性菧类成分longistylin A与longistylin C为评价指标的树豆(木豆)药材质量控制方法.方法:采用硅胶柱色谱分离纯化有关成分,光谱技术鉴定化学结构;反相高效液相色谱法测定指标成分的含量.结果:从树豆叶分离鉴定了2种指标成分longistylin A和longistylin C;采用Thermo BDS Hypersil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-水(80:20)为流动相可使指标成分获得良好分离;longistylin A和longistylin C分别在0.002 88～0.057 6μg和0.011 2～0.224 μg呈良好的线性关系,平均回收率分别为98.9％,97.2％,RSD分别为2.4％,2.2％.结论:建立了以树豆叶中药物活性菧类成分为指标的药物分析方法,可用于树豆(木豆)药材的质量控制. |
| 关 键 词： | 树豆 longistylin A longistylin C 结构鉴定 高效液相色谱 |
| 收稿时间： | 2010/12/20 0:00:00 |
Determination of longistylin A and longistylin C in Cajanus cajan |
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| WANG Dawu,XIAO Minxun,LI Yirong,SHEN Xiaoling,LU Yuanyuan,LIU Kanglun,LI Zhenghong and HU Yingjie.Determination of longistylin A and longistylin C in Cajanus cajan[J].China Journal of Chinese Materia Medica,2011,36(19):2680-2683. | |
| Authors: | WANG Dawu XIAO Minxun LI Yirong SHEN Xiaoling LU Yuanyuan LIU Kanglun LI Zhenghong and HU Yingjie |
| Affiliation: | Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Herbarium of National research Institute of Chinese Medicine, Taipei, China;Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China;Institute of Resources Insects of the Chinese Academy of Forestry, Kunming 650224, China;Tropical Medicine Institute, Guangzhou University of Chinese Medicine, Guangzhou 510405, China |
| Abstract: | Objective: To establish quality control criteria for medicinal herb Cajanus cajan based on the determination of longistylin A and longistylin C,two bioactive and specific stilbenes of the plant.Method: Longistylin A and longistylin C were obtained from the leaves of C.cajan by silica gel column chromatography and identified as marker compounds of this plant by spectroscopic analysis.A RP-HPLC method was established to determine the two compounds.Result: Longistylin A and longistylin C were well separated on... |
| Keywords: | Cajanus cajan longistylin A longistylin C structural identification HPLC |
| 本文献已被 CNKI 万方数据 PubMed 等数据库收录！ | |
