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| 细辛非挥发性提取物抗炎镇痛作用研究 | |
| 引用本文： | 熊玉兰,荆宇,尚明英,李长龄,叶加,王璇,蔡少青.细辛非挥发性提取物抗炎镇痛作用研究[J].中国中药杂志,2009,34(17):2252-2257. |
| 作者姓名： | 熊玉兰 荆宇 尚明英 李长龄 叶加 王璇 蔡少青 |
| 作者单位： | 1. 北京大学药学院分子与细胞药理学系,北京,100191 2. 北京大学药学院天然药物学系,北京,100191 3. 北京大学药学院化学生物学系,北京,100191 |
| 基金项目： | 国家科技支撑计划(2006BAI14B01-3)；国家重点基础研究发展计划(973)项目(2006CB504707) |
| 摘 要： | 目的:考察细辛不同煎煮时间的水煎液及不同浓度乙醇提取物是否具有抗炎及镇痛作用,并初步探讨细辛的抗炎镇痛作用机制.方法:采用ICR小鼠,以小鼠耳肿胀法对北细辛(根和根茎)水煎液(提取挥发油后水煎液和临床常用水煎液)和乙醇提取物(95%乙醇提取物和50%乙醇提取物)进行抗炎作用的评价;以热板法对北细辛水煎液进行镇痛作用的评价;并以去肾上腺试验和纳洛酮阻断试验,探讨细辛水煎液的抗炎镇痛机制.结果:二甲苯小鼠耳肿胀试验中,北细辛2种水煎液明显减轻炎症肿胀度,高剂量时抑制率分别为43.20%,和63.69%,而对二甲苯所致去肾上腺小鼠耳肿胀无抑制作用;北细辛95%乙醇提取物和50%乙醇提取物均能抑制小鼠耳肿胀,高剂量时抑制率分别为61.86%和52.56%.在热板试验中,北细辛2种水煎液高、低剂量均使痛反应潜伏期明显延长,镇痛作用在2.0 h达高峰;2.0 h各组痛阈提高率分别为51.27%,62.78%,60.08%和68.00%;经纳洛酮预处理后潜伏期明显缩短,北细辛水煎液未显示镇痛作用.结论:北细辛水煎液有明显的抗炎和镇痛作用.临床常用水煎液的抗炎镇痛作用强于提取挥发油后的水煎液.北细辛乙醇提取物具有显著的抗炎作用,95%乙醇提取物抗炎作用与50%乙醇提取物相当.北细辛水煎液抗炎作用有肾上腺的参与,镇痛作用与阿片受体有关. |
| 关 键 词： | 细辛水煎液 细辛乙醇提取物 抗炎 镇痛 |
| 收稿时间： | 4/2/2009 12:00:00 AM |
Anti-inflammatory and anti-nociceptive effects in mice of water and ethanol extracts of roots and rhizomes of Asarum heterotropoides var. mandshuricum |
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| XIONG Yulan,JING Yu,SHANG Mingying,LI Changling,YE Ji,WANG Xuan and CAI Shaoqing.Anti-inflammatory and anti-nociceptive effects in mice of water and ethanol extracts of roots and rhizomes of Asarum heterotropoides var. mandshuricum[J].China Journal of Chinese Materia Medica,2009,34(17):2252-2257. | |
| Authors: | XIONG Yulan JING Yu SHANG Mingying LI Changling YE Ji WANG Xuan and CAI Shaoqing |
| Abstract: | Objective: To compare the anti-inflammatory and anti-nociceptive effects of the different water extracts which were prepared by regular decoction time or removing volatie oil and ethanol extracts which were prepared in different concentration ethanol of Xixin (the roots and rhizomes of Asarum heterotropoides var. mandshuricum), and then to investigate the anti-inflammatory and anti-nociceptive mechanisms. Method: Xylene-induced ICR mouse ear edema and hot plate test were utilized to evaluate the anti-inflammatory and anti-nociceptive effects of Xixin at different dose level (water extracts of regular decoction time or removing volatie oil at the dose of 0.8 g·kg-1 and 1.6 g·kg-1; 95% ethanol extracts at the dose of 0.91 g·kg-1 and 1.82 g·kg-1; 50% ethanol extracts at the dose of 0.76 g·kg-1 and 1.52 g·kg-1). Xylene-induced adrenalectomized mouse ear edema model was used to study the anti-inflammatory mechanisms. To reveal the anti-nociceptive mechanisms, mice were pretreated with naloxone in the hot plate test. Result: The water extracts significantly reduced the weight of ear edema as compared with control group. Inhibition ratios were 43.20% and 63.69% at the higher dose, respectively. The ethanol extracts also significantly reduced the weight of ear edema and the inhibition ratios were 61.86% and 52.56% at the higher dose, respectively. Mice administered water extracts delayed the latency times in the hot plate test. The anti-nociceptive effects of water extracts peaked at 2.0 h after i.g. administration. The hot plate latency times were increased by 51.27%, 62.78%, 60.08% and 68.00% at peak time, respectively. Regular decoction time group showed more significant effects in both models. The anti-inflammatory effect of 95% ethanol extracts was similar to 50% ethanol extracts. The water extracts were not effective in reducing xylene-induced adrenalectomized mouse ear edema. The anti-nociceptive effect of water extracts was blocked by naloxone. Conclusion: Both the water extracts and ethanol extracts of Xixin showed considerable anti-inflammatory potency against xylene induced inflammation. The water extracts produced anti-nociception in thermal model. The water extracts prepared in regular decoction time showed better anti-inflammatory and anti-nociceptive effects. Both the 95% ethanol and 50% ethanol extracts showed similarly anti-inflammatory effects. The anti-inflammatory effect of water extracts related to adrenal gland. The anti-nociceptive effect of water extracts was involved in activating opioid receptor. |
| Keywords: | Xixin Asarum heterotropoides var mandshuricum water extracts ethanol extracts anti-inflammation anti-nociception |
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