| 三叶木通愈伤组织培养研究 |
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| 引用本文： | 沈国林,邵爱娟 黄璐琦,林淑芳.三叶木通愈伤组织培养研究[J].中国中药杂志,2007,32(10):899-901. |
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| 作者姓名： | 沈国林 邵爱娟 黄璐琦 林淑芳 |
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| 作者单位： | 中国中医科学院,中药研究所,北京,100700 |
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| 摘 要： | 目的：探讨影响三叶木通愈伤组织诱导因素,建立三叶木通愈伤组织培养方法。方法：采用组织培养方法,比较不同外植体、培养基、pH、温度、光照、植物生长物质种类及其配比对三叶木通愈伤组织诱导的影响。结果：叶片的诱导率最高,为87.5%；茎段次之,叶柄较差；培养基MS,B5愈伤组织诱导率较高,达到了80%以上,高于培养基H,SH和改良White的愈伤组织诱导率；偏低的pH对愈伤组织生长有利,出愈率高,但从愈伤组织生长质量方面看,不同pH差异不明显；愈伤组织诱导最适温度为25 ℃。结论：三叶木通愈伤组织的适宜培养基为MS+2,4-D 4.0 mg·L-1+NAA 1.0 mg·L-1+KT 1.0 mg·L-1(pH 5.8),培养温度为25 ℃,暗培养。
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| 关 键 词： | 三叶木通 愈伤组织 诱导 |
| 文章编号： | 1001-5302（2007）10-0899-03 |
| 收稿时间： | 2006-06-20 |
| 修稿时间： | 2006-06-20 |
Studies on callus culture of Akebia trifoliata |
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| SHEN Guo-lin; SHAO Ai-juan; HUANG Lu-qi; LIN Shu-fang.Studies on callus culture of Akebia trifoliata[J].China Journal of Chinese Materia Medica,2007,32(10):899-901. |
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| Authors: | SHEN Guo-lin; SHAO Ai-juan; HUANG Lu-qi; LIN Shu-fang |
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| Affiliation: | Institute of Chinese Materia Medica, Academy of Chinese Medical Sciences, Beijing 100700, China |
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| Abstract: | OBJECTIVE: To investigate the inductive factors of effect on Akebia trifoliata and establish culture method for A. trifoliata callus. METHOD: To study the possible effective factors of culture condition by comparing with different explantation, nutrient medium, pH, temperature, illumination, growth substance of plant and its ratio. RESULT: The inductivity of leaves was the highest about 87.5%, followed with the stem section and leafstalk; The inductivity of nutrient medium such as MS, B5 callus was higher than the ones like H, SH and the White callus amended one; It was found that low-grade Phvalue benefits the growth of callus. The experiment result showed that different pH showed little difference in quality. The best condition of culture was 25 degrees C in temperature. CONCLUSION: The best culture condition for callus was the leaves as explantation. The A. trifoliata callus culture's best inductive condition was MS +2, 4-D 4.0 mg x L(-1) + NAA 1.0 mg x L(-1) + KT 1.0 mg x L(-1) (pH 5.8), cultural temperature was 25 degrees C, cultivation was dark. |
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| Keywords: | Akebia trifoliata callus induction |
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