| 蝎毒多肽提取物对小鼠Lewis肺癌免疫逃逸的影响 |
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| 引用本文： | 徐林,张维东,王兆朋,贾青,张月英,姜国胜.蝎毒多肽提取物对小鼠Lewis肺癌免疫逃逸的影响[J].中国中药杂志,2010,35(17):2324-2327. |
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| 作者姓名： | 徐林 张维东 王兆朋 贾青 张月英 姜国胜 |
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| 作者单位： | 山东省医学科学院,基础所,实验病理学与生理学研究室,山东现代医用药物与技术重点实验室,山东,济南,250062 |
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| 基金项目： | 国家自然科学基金项目(30873408);山东省中药现代化重大科技计划项目(2008GG2NS02023);山东省自然科学基金项目(Y2007C094,Y2008C176,ZR2009CL030);山东省科技攻关项目(2008GG30002067) |
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| 摘 要： | 目的:观察蝎毒多肽提取物(PESV)对Lewis肺癌(LLC)免疫逃逸的影响。方法:取40只C57BL/6J小鼠,右腋下接种Lewis肺癌细胞悬液,建立皮下种植瘤模型,随机分为荷瘤对照组和PESV治疗组,连续灌胃给予PESV 18 d,检测肿瘤体积并计算抑瘤率;免疫组化法和ELISA法分别检测瘤组织及血清中血管内皮生长因子(VEGF)、转化生长因子β1(TGF-β1)和白介素10(IL-10)的表达,免疫组化法和流式细胞仪分别检测肺癌组织中浸润的DC表面共刺激分子CD80,CD86的表达。结果:PESV抑瘤率为56.60%。与荷瘤对照组相比,瘤组织和血清中VEGF,TGF-β1和IL-10的表达明显降低(P0.05),DC共刺激分子CD80,CD86的表达明显增加(P0.05)。结论:PESV可干预肺癌免疫逃逸,其机制可能与减少肿瘤微环境中VEGF,TGF-β1和IL-10的表达,增加DC共刺激分子CD80,CD86的表达有关。
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| 关 键 词： | 蝎毒液类 肺肿瘤 肿瘤逃逸 |
| 收稿时间： | 2010/1/28 0:00:00 |
Effect of polypeptide extract from scorpion venom (PESV) on immune escape of Lewis lung carcinomas |
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| XU Lin,ZHANG Weidong,WANG Zhaopeng,JIA Qing,ZHANG Yueying and JIANG Guosheng.Effect of polypeptide extract from scorpion venom (PESV) on immune escape of Lewis lung carcinomas[J].China Journal of Chinese Materia Medica,2010,35(17):2324-2327. |
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| Authors: | XU Lin ZHANG Weidong WANG Zhaopeng JIA Qing ZHANG Yueying and JIANG Guosheng |
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| Affiliation: | Department of Experimental Pathology and Pathophysiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Modern Medicine and Technology of Shandong Province, Jinan 250062, China;Department of Experimental Pathology and Pathophysiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Modern Medicine and Technology of Shandong Province, Jinan 250062, China;Department of Experimental Pathology and Pathophysiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Modern Medicine and Technology of Shandong Province, Jinan 250062, China;Department of Experimental Pathology and Pathophysiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Modern Medicine and Technology of Shandong Province, Jinan 250062, China;Department of Experimental Pathology and Pathophysiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Modern Medicine and Technology of Shandong Province, Jinan 250062, China;Department of Experimental Pathology and Pathophysiology, Institute of Basic Medicine, Shandong Academy of Medical Sciences, Key Laboratory of Modern Medicine and Technology of Shandong Province, Jinan 250062, China |
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| Abstract: | |
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| Keywords: | scorpion venoms lung neoplasms tumor escape |
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