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| AFLP分析唐古特大黄、掌叶大黄和药用大黄的亲缘关系研究 | |
| 引用本文： | 索风梅,宋经元,陈士林,王永刚,梅红芳,EijiMiki,OsamiTake,谢彩香.AFLP分析唐古特大黄、掌叶大黄和药用大黄的亲缘关系研究[J].中草药,2010,41(2):292-296. |
| 作者姓名： | 索风梅 宋经元 陈士林 王永刚 梅红芳 EijiMiki OsamiTake 谢彩香 |
| 作者单位： | 1. 中国医学科学院北京协和医学院药用植物研究所,北京,100193 2. 中国医药保健品股份有限公司,北京,100061 3. Botanical Raw Materials Research Department,Botanical Raw Materials Division,Tsumura & Co.3586 Yoshiwara,Ami-machi,Inashiki-gun,Ibaraki 300-1192,Japan |
| 基金项目： | 国家中医药管理局中医药行业科研专项(200707007); 中国医药保健品股份有限公司、日本津村与中国医学科学院药用植物研究所合作项目资助 |
| 摘 要： | 目的研究《中国药典》2005年版收载蓼科大黄属3种大黄(唐古特大黄、掌叶大黄和药用大黄)的亲缘关系。方法对3种大黄44份样品进行AFLP分析,同时利用NTSYSpc-2.11F软件分析其亲缘关系。结果8对选择性扩增引物中,扩增得到1 255条稳定清晰的条带,其中多态性条带1 209条,多态性带占总带数的96.2%。将44份大黄样品分为2组2个亚组。四川若尔盖唐古特大黄聚为第一组,第二组分为两个亚组,分别为药用大黄和掌叶大黄。另外,大黄的AFLP分子标记谱带显示,3种大黄和不同来源的大黄样品间除了具有共有带外,还有一些特异带,说明种间甚至不同来源的样品间有一定程度的特异性。结论《中国药典》规定的3种正品大黄中,药用大黄和掌叶大黄亲缘关系较唐古特大黄近,表明利用AFLP技术分析3种大黄的亲缘关系可行。 |
| 关 键 词： | 大黄 AFLP分析 亲缘关系 蓼科 大黄属 |
| 收稿时间： | 2009/5/20 0:00:00 |
AFLP Analysis on genetic relationship among Rheum tanguticum, Rheum palmatum, and Rheum officinale |
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| SUO Feng-mei,SONG Jing-yuan,CHEN Shi-lin,WANG Yong-gang,MEI Hong-fang,EIJI Miki,OSAMI Takeda and XIE Cai-xiang.AFLP Analysis on genetic relationship among Rheum tanguticum, Rheum palmatum, and Rheum officinale[J].Chinese Traditional and Herbal Drugs,2010,41(2):292-296. | |
| Authors: | SUO Feng-mei SONG Jing-yuan CHEN Shi-lin WANG Yong-gang MEI Hong-fang EIJI Miki OSAMI Takeda and XIE Cai-xiang |
| Affiliation: | SUO Feng-mei1,SONG Jing-yuan1,CHEN Shi-lin1,WANG Yong-gang2,MEI Hong-fang2,EIJI Miki3,OSAMI Takeda3,XIE Cai-xiang1(1.Institute of Medicinal Plant Development,Chinese Academy of Medical Sciences,Chinese Peking Union Medical College,Beijing 100193,China,2.China Meheco Corporation,Beijing 100061,3.Botanical Raw Materials Research Department,Botanical Raw Materials Division,Tsumura & Co.3586 Yoshiwara,Ami-machi,Inashiki-gun,Ibaraki 300-1192,Japan) |
| Abstract: | Objective To investigate the genetic relationship of three traditional medicinal rhubarbs(Rheum tanguticum,R.palmatum,and R.officinale.Methods Amplified fragment length polymorphism(AFLP) technology and NTSYSpc-2.11F software were used to study and analyze the affinity between 44 wild or cultivated medicinal rhubarbs from different provenances.Results The 1 255 bands were amplified by polymerase chain reactions with eight screened and reliable primers.Among them 1 209(96.2%) bands showed polymorphic.The 44 ... |
| Keywords: | rhubarb amplified fragment length polymorphism(AFLP) genetic relationship Polygonaceae Rheum L |
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