| 中药及其成分的生物转化 |
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| 引用本文： | 马百平,冯 冰,黄鸿志,从玉文.中药及其成分的生物转化[J].世界科学技术-中医药现代化,2010,12(2):150-154. |
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| 作者姓名： | 马百平 冯 冰 黄鸿志 从玉文 |
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| 作者单位： | 军事医学科学院放射与辐射医学研究所 北京 100850 |
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| 基金项目： | 国家自然科学基金面上项目（30572333）：中药呋甾皂苷3-糖链中糖基的选择性水解研究，负责人：马百平；国家自然科学基金面上项目（20672142）：三种葡萄糖醛酸苷酶的结构及其作用专一性研究，负责人：马百平；国家自然科学基金面上项目（30973632）：甾体皂苷糖基化的生物转化研究，负责人：马百平；北京市科委北京市自然科学基金重大项目（7090001）：基于化学基因组学的甾体皂苷药靶发现与结构优化研究，负责人：马百平；中国博士后科学基金会中国博士后科学基金（20070410510）：新月弯孢霉中特异性鼠李糖苷酶研究及鼠李糖在甾体皂苷中的药理学意义，负责人：冯冰。 |
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| 摘 要： | 生物转化已在中药的发酵炮制、中药代谢、有效成分结构修饰与合成中得到了应用。我们以中药甾体皂苷为研究对象，系统进行甾体皂苷的糖基选择性水解、糖基化以及皂苷元的结构修饰，寻找一些特异性微生物和酶，探讨生物转化进行甾体皂苷结构修饰的规律性。这些深入研究可以为确定中药有效成分的活性中心、进行定向结构修饰、构建具有结构多样性的聚焦化合物库、进行新药开发目标化合物的产业化制备奠定良好的基础，也可为本草物质资源库和本草药效物质基础研究提供有价值的策略和方法。
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| 关 键 词： | 中药 甾体皂苷 生物转化 糖苷酶 结构修饰 |
| 收稿时间： | 2009/10/12 0:00:00 |
| 修稿时间： | 2009/10/13 0:00:00 |
Biotransformation of Chinese Herbs and Their Ingredients |
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| Ma Baiping,Feng Bing,Huang Hongzhi and Cong Yuwen.Biotransformation of Chinese Herbs and Their Ingredients[J].World Science and Technology-Modernization of Traditional Chinese Medicine,2010,12(2):150-154. |
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| Authors: | Ma Baiping Feng Bing Huang Hongzhi and Cong Yuwen |
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| Affiliation: | Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850, China |
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| Abstract: | Biotransformation has been extensively applied in herbal fermentation processing, metabolism study of Traditional Chinese Medicines (TCMs), structural modification and synthesis of active components of TCMs. Selecting steroidal saponins as the subject, the selective hydrolysis of glycosyl residues, glycosylation of molecules, and the modification of their aglycone were systematically studied by biotechnology in this work. Some active microorganisms were screened, and several specific enzymes were purified. Their substrate specificities and transformed regularities were summarized. These results lay a better foundation for determining the key core of the active components, specifically modifying their structures, constructing the focused library with the structural diversity, and manufacturing some new drug candidates which are rare in herbs. They may also provide a kind of valuable strategy and approach support for herbal substance library construction as well as the active ingredients research of Herbalome Project. |
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| Keywords: | Traditional Chinese Medicine Steroidal saponin Biotransformation Glycosidase Structural modification |
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