| 低频脉冲磁场调节NO/ONOO-对缺氧/复氧条件下心肌细胞的保护作用 |
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| 引用本文： | 张正勋,易甫,范伟伟,张荣庆,王东娟,李秀娟,曹丰.低频脉冲磁场调节NO/ONOO-对缺氧/复氧条件下心肌细胞的保护作用[J].心脏杂志,2013,25(2):134-139. |
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| 作者姓名： | 张正勋 易甫 范伟伟 张荣庆 王东娟 李秀娟 曹丰 |
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| 作者单位： | (第四军医大学西京医院心脏内科，陕西 西安 710032) |
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| 基金项目： | 国家自然科学基金项目资助(81000686) |
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| 摘 要： | 目的:观察低频脉冲磁场（LF-PMFs）对缺氧/复氧（H/R）条件下乳鼠心肌细胞增殖与凋亡的影响，并探讨一氧化氮/过氧亚硝基阴离子（NO/ONOO-）平衡在LF-PMFs保护心肌细胞的作用。方法: 采用酶消化法分离SD乳鼠心肌细胞，随机分为对照组、H/R组和磁场组。用TUNEL法检测心肌细胞凋亡程度，MTT比色法检测心肌细胞增殖活性，以观察LF-PMFs对损伤心肌细胞的效果，用WST-1 法检测超氧阴离子，Griess法测定NO和ONOO-。结果: LF-PMFs磁场可减少H/R诱导的心肌细胞凋亡，并促进心肌细胞的存活，以15 Hz，4.5 mT LF-PMFs磁场的作用效果最为显著。同时LF-PMFs可减少H/R条件下乳鼠心肌细胞超氧阴离子的生成，促进NO生成，提高NO/ONOO-比值。结论: LF-PMFs可通过抗氧化及调节NO/ONOO-平衡减轻H/R导致的心肌细胞损伤。
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| 关 键 词： | 低频脉冲磁场 心肌细胞 缺氧/复氧 NO/ONOO-平衡 |
| 收稿时间： | 2012-11-12 |
Low-frequency pulsed magnetic fields protect cardiomyocytes from hypoxia/reoxygenation injury by affecting the “NO/ONOO- balance” |
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| ZHANG Zheng-xun,YI Fu,FAN Wei-wei,ZHANG Rong-qing,WANG Dong-juan,LI Xiu-juan,CAO Feng.Low-frequency pulsed magnetic fields protect cardiomyocytes from hypoxia/reoxygenation injury by affecting the “NO/ONOO- balance”[J].Chinese Heart Journal,2013,25(2):134-139. |
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| Authors: | ZHANG Zheng-xun YI Fu FAN Wei-wei ZHANG Rong-qing WANG Dong-juan LI Xiu-juan CAO Feng |
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| Affiliation: | (Department of Cardiology,Xijing Hospital,Fourth Military Medical University,Xi’ an 710032,Shaanxi,China) |
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| Abstract: | AIM:To investigate the effect of low-frequency pulsed magnetic fields (LF-PMFs) on proliferation and apoptosis of neonatal cardiomyocytes after hypoxia/reoxygenation (H/R) injury and the mechanism of NO/ONOO- balance involved. METHODS: Cultured neonatal cardiomyocytes were randomized into three groups: control group, H/R group, and LF-PMFs+H/R group. Viability and apoptosis of cardiomyocytes were assessed by MTT and TUNEL and levels of superoxide anion and NO/ONOO- were tested by Superoxide Assay Kit and Griess Assay, respectively. RESULTS: LF-PMFs promoted proliferation and inhibited apoptosis of neonatal cardiomyocytes following H/R treatment. Pre-/pro-treatment with LF-PMFs significantly downregulated the level of superoxide anion and ONOO- and upregulated NO expression of cardiomyocytes in vitro. LF-PMFs with 15 Hz, 4.5 mT yielded the most beneficial effect compared with magnetic fields at other levels. CONCLUSIONS: LF-PMFs exert protective effects on cardiomyocytes from H/R injury via balancing NO/ONOO-. |
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