| 芪参益气滴丸减轻心肌缺血再灌注损伤的作用 |
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| 引用本文： | 何贵新,冯雨菲,玉黎燕,肖婷,秦伟彬,吴成强,申永艳,胡梦弦,郑国坤.芪参益气滴丸减轻心肌缺血再灌注损伤的作用[J].心脏杂志,2021,33(3):233-238. |
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| 作者姓名： | 何贵新 冯雨菲 玉黎燕 肖婷 秦伟彬 吴成强 申永艳 胡梦弦 郑国坤 |
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| 作者单位： | 1.第一附属医院心内科 广西中医药大学 |
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| 基金项目： | 国家自然科学基金项目资助（81460712）；广西研究生教育创新计划项目资助（YCSY2020099） |
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| 摘 要： | 目的 探究芪参益气滴丸减轻巴马猪急性心肌缺血再灌注损伤的作用以及其对心肌蛋白激酶B(AKT) 和磷酸化蛋白激酶B(p-AKT) 蛋白表达的影响。 方法 将20只健康广西巴马猪（巴马猪）随机分为:模型组和芪参益气滴丸组，每组10只（n=10）。两组巴马猪正常喂食外，模型组予100 ml纯净水灌胃，芪参益气滴丸组予芪参益气滴丸+100 ml纯净水灌胃，3次/d，持续2个月。结扎巴马猪左冠状动脉前降支，建立心肌缺血再灌注损伤模型。ELISA法检测血清肌酸激酶（CK）、肌酸激酶同工酶（CK-MB）、肌钙蛋白T（cTnT）；2,3,5-氯化三苯基四氮唑（TTC）染色检测心肌梗死的区域的面积；Western blot法检测心肌组织正常区、边缘区、梗死区内AKT和P-AKT蛋白表达水平。 结果 ①芪参益气滴丸组CK、CK-MB、cTnT值均小于模型组（P<0.05）；②与模型组比较，芪参益气滴丸组巴马猪心肌梗死面积明显减小，差异有统计学意义（P<0.05）；③与模型组比较，芪参益气滴丸组AKT蛋白表达水平无明显变化，而P-AKT蛋白表达水平明显增加，差异有统计学意义（P<0.01）。 结论 芪参益气滴丸可缩小梗死心肌面积，减轻心肌损伤标记物的含量，进而缓解心肌组织的缺血再灌注损伤，同时激活心肌组织总体p-AKT蛋白的表达。
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| 关 键 词： | 芪参益气滴丸 心肌缺血再灌注 磷脂酰肌醇3 激酶 蛋白激酶B |
| 收稿时间： | 2021-03-21 |
Qishen Yiqi Dropping Pill alleviates myocardial ischemia-reperfusion injury and its effect on the expression of AKT and p-AKT protein in myocardial tissue |
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| Affiliation: | 1.Department of Cardiology, First Affiliated Hospital2.Graduate School, Guangxi University of Traditional Chinese Medicine, Nanning 530022, Guangxi, China |
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| Abstract: | AIM To investigate the effects of Qishen Yiqi Dropping Pills on alleviating acute myocardial ischemia-reperfusion injury in Bama pigs and its effects on the expression of protein kinase B(Akt) and phosphorylated protein kinase B(p-Akt). METHODS Twenty healthy Guangxi Bama pigs were randomly divided into Qishen Yiqi Dropping Pill group (n = 10) and model group (n = 10). In addition to the normal feeding of Bama pigs in the two groups, Qishen Yiqi Dropping Pills group was given Qishen Yiqi Dropping Pills plus 100 ml pure water, while the model group was given 100 ml pure water by intragastric administration, 3 times a day for 2 months. The model of myocardial ischemia-reperfusion injury was established by ligating the anterior descending branch of the left coronary artery in Bama pigs. Serum CK, CK-MB and cTnT 2 were detected by ELISA method, the area of myocardial infarction was detected by triphenyltetrazolium chloride (TTC) staining and the expression levels of AKT and P-AKT in normal zone, marginal zone and infarcted area were detected by Western blot. RESULTS ELISA showed that CK, CK-MB and cTnT values in Qishen Yiqi Qropping Pill group were all smaller than those in model group (P<0.05). TTC staining found that compared with those in model group, the area of myocardial infarction in Bama pigs in Qishen Yiqi Dropping Pills group was significantly reduced and the difference was statistically significant (P<0.05). Western blot detection results showed that no significant changes in the expression level of AKT protein were seen between model group and Qishen Yiqi Dropping Pills group, but the expression of P-AKT protein in Qishen Yiqi Dropping Pills group increased significantly and the difference was statistically significant (P<0.01). CONCLUSION Qishen Yiqi Dropping Pills reduces the area of infarted myocardium and the content of markers of myocardial injury, and then alleviates the ischemia reperfusion injury of myocardial tissue, meanwhile, it activates the expression of p-Akt protein in cardiac tissue. |
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