| 红景天苷对高海拔缺氧条件下大鼠心肌自噬相关通路AMPK的影响 |
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| 引用本文： | 杜丽,马玲,王楠.红景天苷对高海拔缺氧条件下大鼠心肌自噬相关通路AMPK的影响[J].心脏杂志,2022,34(4):378-382. |
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| 作者姓名： | 杜丽 马玲 王楠 |
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| 作者单位： | 1.甘肃医学院附属医院心内科, 甘肃 平凉 744000 |
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| 基金项目： | 甘肃省人民医院重点学科项目资助（18GSGY1-3） |
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| 摘 要： | 目的 探讨红景天苷保护高海拔缺氧损伤大鼠心脏的可能分子机制。 方法 将40只Wistar大鼠随机分为5组:空白对照组（1 500 m）、缺氧生理盐水组（6 500 m+NS）、缺氧Compound C组（6 500 m+comC）、缺氧红景天苷组（6 500 m +Sal）、缺氧红景天苷+Compound C组（6 500 m +comC+Sal）。造模完成后进行血气分析、HE染色、荧光TUNEL检测及Western blot检测。 结果 ①缺氧后大鼠心肌结构发生变化，心肌凋亡加重（P<0.05），红景天苷治疗可改善心肌细胞病理性损伤，减少心肌细胞凋亡（P<0.05）；②缺氧后磷酸化AMPK水平上升，磷酸化mTOR水平降低（P<0.05）。而Compound C组AMPK及mTOR表现呈相反趋势，红景天苷治疗后AMPK及mTOR的表达水平介于Compound C组与红景天苷组之间，但是自噬蛋白Beclin-1的表达较Compound C组增加（P<0.05）。 结论 红景天苷可以正向调节AMPK信号通路激活自噬，对缺氧损伤的大鼠心脏起保护作用。
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| 关 键 词： | AMPK 缺氧 红景天苷 信号通路 自噬 |
| 收稿时间： | 2020-09-27 |
Effects of salidroside on AMPK in myocardial autophagy related pathway under simulated high altitude hypoxia |
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| Affiliation: | 1.Department of Cardiology, Affiliated Hospital of Gansu Medical College, Ping Liang 744000, Gansu, China2.Department of Cardiology, Gansu Provincial People’s Hospital, Lanzhou 730000, Gansu, China |
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| Abstract: | AIM To investigate the molecular pharmacological mechanism of Salidroside in protecting rat heart injury induced by high altitude hypoxia. METHODS Wistar rats were randomly divided into 5 groups: blank control group (1 500 m), hypoxic saline group (6 500 m+NS), hypoxia compound C group (6 500 m+comC), hypoxia salidroside group（6 500 m+Sal）, hypoxia salidroside+ compound C group (6 500 m+comC+Sal). RESULTS After exposure to hypoxic environment, myocardial structure was changed and myocardial apoptosis was aggravated（P<0.05）. Salidroside treatment could improve the pathological damage of myocardial cells and reduce the degree of myocardial apoptosis（P<0.05）; At the same time, the level of phosphorylated AMPK increased and the level of phosphorylated mTOR decreased（P<0.05）. The expression levels of AMPK and mTOR were between compound C group and salidroside group, but the expression of autophagy protein Beclin-1 was higher than that of compound C group（P<0.05）. CONCLUSION salidroside can positively regulate AMPK signaling pathway, activate autophagy and protect myocardium from hypoxia injury. |
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