A Platelet-Dependent Serum Factor That Stimulates the Proliferation of Arterial Smooth Muscle Cells In Vitro |
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| Authors: | Russell Ross John Glomset Beverly Kariya and Laurence Harker |
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| Affiliation: | *University of Washington, School of Medicine, Department of Pathology, Seattle Wash. 98195;†University of Washington, School of Medicine, Department of Medicine, Seattle, Wash. 98195;Regional Primate Research Center, Seattle, Wash. 98195;‡University of Washington, School of Medicine, Department of Medicine, Seattle, Wash. 98195 |
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| Abstract: | Dialyzed serum from clotted monkey blood ("blood serum") promotes the proliferation of monkey arterial smooth muscle cells in culture, but dialyzed serum prepared from recalcified platelet-poor plasma ("plasma serum") is much less effective. Addition of platelets and calcium to platelet-poor plasma increases the activity of plasma serum to the same level achieved with blood serum. Furthermore, addition to plasma serum of a platelet-free supernatant prepared by exposing purified platelets to thrombin also stimulates the proliferation of smooth muscle cells. Thus, much of the growth-promoting activity of dialyzed serum is directly or indirectly derived from platelets. This finding has important implications for the response of arteries to localized injury and provides a key to further understanding of the role of factors derived from blood serum in promoting cell proliferation in vitro. |
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