| 基于线粒体基因分析的中华血吸虫分子种系发生研究 |
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| 引用本文： | 张广军,邱持平,邱东川,夏明仪.基于线粒体基因分析的中华血吸虫分子种系发生研究[J].中国寄生虫学与寄生虫病杂志,2002,20(1):10-13. |
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| 作者姓名： | 张广军 邱持平 邱东川 夏明仪 |
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| 作者单位： | 1. 中国疾病预防控制中心寄生虫病预防控制所,世界卫生组织疟疾、血吸虫病和丝虫病合作中心,卫生部病原与媒介生物学重点实验室,上海200025
2. 四川省寄生虫病研究所,成都610041 |
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| 基金项目： | 卫生部科学研究基金资助项目 (No.97- 1 - 0 2 3)~~ |
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| 摘 要： | 目的 测定中华血吸虫线粒体细胞色素 C氧化酶亚基 1(CO1)和 NADH脱氢酶亚基 1(ND1)基因序列 ,并根据这些序列构建分子系统发生树 ,探讨中华血吸虫在裂体属内的系统发生位置。 方法 以 GNT- K法抽提虫体基因组 DNA,用特异引物 PCR扩增目的基因。PCR扩增产物经纯化后克隆于质粒载体 ,以纯化后的阳性质粒 DNA作为模板 ,M13(F/ R)为引物于 L icor测序仪测序。检索 Gen Bank,查找曼氏血吸虫等相关血吸虫两线粒体基因序列 ,作基因排序及比较分析后 ,用 PHYL IP和 MEGA以邻接法和最大简约法绘制系统发生树。 结果 克隆了中华血吸虫的 CO1和 ND1基因片段 ,并测定了两基因片段的核苷酸序列 ,根据这些序列构建了系统发生树。 结论 中华血吸虫 CO1和ND1基因的系统发生树结果一致。提示中华血吸虫归属于亚洲血吸虫组
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| 关 键 词： | 中华血吸虫 CO1 ND1 系统发生分析 |
| 文章编号： | 1000-7423(2002)-01-0010-04 |
| 修稿时间： | 2001年4月6日 |
Study on Molecular Phylogeny of Schistosoma sinensium Based on Mitochondrial Genes |
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| ZHANG Guangjun,QIU Chiping,QIU Dongchuan,XIA Mingyi.Study on Molecular Phylogeny of Schistosoma sinensium Based on Mitochondrial Genes[J].Chinese Journal of Parasitology and Parasitic Diseases,2002,20(1):10-13. |
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| Authors: | ZHANG Guangjun QIU Chiping QIU Dongchuan XIA Mingyi |
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| Affiliation: | Institute for Parasitic Disease Control and Prevention, Chinese Center for Disease Control and Prenention, Shanghai 200025. |
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| Abstract: | Objective To determine the phylogenetic position of Schistosoma sinensium in the genus Schistosoma using mitochondrial cytochrome C oxidase 1 (CO1) and NADH dehydrogenase 1(ND1) as molecular markers. Methods The genomic DNA of adult worms were extracted by the GNT\|K method. The target regions were amplified by PCR using specific primers. The PCR products were purified before ligation into the plasmid Zero\|Blunt. Recombinant plasmids were amplified in E.coli, extracted and purified using routine methods and then sequenced using M13 primers (F/R) on a Licor long\|read auto\|sequencer. Sequences of related schistosomes were retrieved from GenBank and aligned with our data in the sequence editor ESEE. Gene trees were constructed in PHYLIP and MEGA using both maximum parsimony and neighbor\|joining methods. For parsimony analysis, all characters were treated as unordered and with equal weights. At least \{3 000\} cycles of bootstrapping were carried out. For analysis in MEGA, all gap columns were deleted. The third position of codon was included. Results The nucleotide and amino acid sequences of CO1 and ND1 of S.sinensium were obtained. Conclusion The phylogenetic trees from these molecular data suggested that S.sinensium belongs to the Asian schistosome group, and the results coincided with the previous rDNA (ITS2 & LSU) analysis results. |
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| Keywords: | Schistosoma sinensium cytochrome C oxidase 1(CO1) NADH dehydrogenase 1 (ND1) phylogenetic analysis |
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