| 非人灵长类恒河猴及食蟹猴与屈光相关的眼球参数研究 |
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| 引用本文： | 何海龙,马雅,牟浩,刘卓琳,金子兵.非人灵长类恒河猴及食蟹猴与屈光相关的眼球参数研究[J].眼科,2022,31(5):349-354. |
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| 作者姓名： | 何海龙 马雅 牟浩 刘卓琳 金子兵 |
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| 作者单位： | 首都医科大学附属北京同仁医院 北京同仁眼科中心 北京市眼科研究所 眼科学与视觉科学北京市重点实验室100730 |
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| 基金项目： | 国家自然科学基金杰出青年科学基金（82125007） |
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| 摘 要： | 目的 对2~5岁恒河猴和食蟹猴的屈光参数进行测量，获得实验用低龄恒河猴及食蟹猴眼部屈光状态相关数值。设计 实验研究。研究对象 自然生长状态下全身发育正常的2~5岁恒河猴24只及食蟹猴12只。方法 超声眼科专用诊断仪进行眼轴长度（AXL）、前房深度（ACD）、晶状体厚度（LT）及玻璃体腔深度（VCD）等生物学参数的测量，睫状肌麻痹（复方托吡卡胺滴眼液）后采用电脑自动验光仪进行屈光度等效球镜（SE）的测量，采用眼底照相及OCT检查除外眼部其他疾病。对所有计量资料进行正态性检验，组间比较采用t检验或Wilcoxon秩和检验。对双眼相关参数与AXL的关联性采用线性混合效应模型分析。主要指标 SE、AXL、ACD、LT、VCD及相关参数与AXL的关联性分析。结果 24只（48眼）恒河猴的SE为（0.14±1.15）D、AXL为19.80（19.18，20.08）mm、ACD为3.65（2.65，3.84）mm，LT为3.93（3.74，4.19）mm，VCD为（12.10±0.69）mm。对于12只（24眼）食蟹猴， SE为（-0.46±0.96）D，AXL为（18.11±0.54）mm，ACD为（3.57±0.32）mm，LT为（3.20±0.20）mm，VCD为（11.34±0.48）mm。恒河猴及食蟹猴左右眼以上参数对比差异均无统计学意义（P均＞0.05）。恒河猴AXL与ACD、VCD及体重存在正相关（β=0.492、0.572、0.550，P均＜0.05）；食蟹猴AXL与ACD及VCD存在正相关（β=0.780、0.876，P均＜0.05）。结论 北京地区自然生长状态下2~5岁恒河猴AXL与ACD、VCD及体重存在正相关，食蟹猴AXL与ACD、VCD存在正相关，可对后续非人灵长类动物模型的构建提供眼部相关屈光参数。（眼科， 2022， 31： 349-354）
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| 关 键 词： | 恒河猴 食蟹猴 等效球镜 眼轴长度 |
| 收稿时间： | 2022-07-24 |
Refractive parameters of rhesus and cynomolgus monkeys in non-human primates |
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| He Hailong,Ma Ya,Mou Hao,Liu Zhuolin,Jin Zibing.Refractive parameters of rhesus and cynomolgus monkeys in non-human primates[J].Ophthalmology in China,2022,31(5):349-354. |
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| Authors: | He Hailong Ma Ya Mou Hao Liu Zhuolin Jin Zibing |
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| Affiliation: | Beijing Institute of Ophthalmology, Beijing Tongren Eye Center, Beijing Tongren Hospital, Capital Medical University, Beijing Key Laboratory of Ophthalmology and Visual Sciences, Beijing 100730, China |
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| Abstract: | Objective To examine the ocular components to refraction of 2~5 years old rhesus monkeys and cynomolgus monkeys. Design Experimental study. Participants Twenty-four rhesus monkeys and 12 cynomolgus monkeys aged 2~5 years old. Methods Axial length (AXL), anterior chamber depth (ACD), lens thickness (LT), vitreous chamber depth (VCD) and spherical equivalent (SE) were measured by A-scan ultrasonography and automatic computer optometry. A nonmydriatic fundus camera and optical coherence tomography were also performed to exclude ocular diseases. Normal distribution data was described as mean ± standard deviation and compared the two groups using t-test. Wilcox on Rank-Sum test was used to compare the continued non-normal distribution data, which was described as median (interquartile range). Given that data of both eyes of monkeys was used, linear mixed-effects model was used to adjust the inter-correlation between right and left eye. Main Outcome Measures SE, AXL, ACD, LT, VCD and correlation analysis between associated parameters and AXL. Results For 24 rhesus monkeys(48 eyes), the SE was (0.14±1.15) D, the AXL was 19.80 (19.18, 20.08) mm, the ACD was 3.65 (2.65, 3.84) mm, the LT was 3.93 (3.74, 4.19) mm, the VCD was 12.10±0.69 mm, there was no significant difference between both eyes (all P＞0.05). For 12 cynomolgus monkeys(24 eyes), the SE was(-0.46±0.96) D, the AXL was (18.11±0.54) mm, the ACD was (3.57±0.32) mm, the LT was (3.20±0.20) mm, the VCD was (11.34±0.48) mm, there was no significant difference between both eyes (all P＞0.05). The AXL of 48 rhesus and 24 cynomolgus monkeys’ eyes were included for linear mixed-effects model. The results of multiple stepwise regression analysis showed that the ACD, VCD, and weight were positively associated with a longer rhesus AXL (β=0.492, 0.572, 0.550, all P<0.05), while ACD and VCD were statistically significantly associated with a longer cynomolgus AXL (β=0.780, 0.876, both P<0.05). Conclusion We found that the ACD, VCD, and weight were positively associated with rhesus axial elongation and ACD and VCD were positively associated with cynomolgus axial elongation in the natural growth development conditions of Beijing, China. The ocular parameters could provide a foundation for future rhesus monkeys and cynomolgus monkeys research. (Ophthalmol CHN, 2022, 31: 349-354) |
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| Keywords: | rhesus monkeys cynomolgus monkeys spherical equivalent axial length |
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