| 松果菊苷对高糖诱导的人视网膜毛细血管内皮细胞增殖、迁移及新生血管形成的影响 |
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| 引用本文： | 毛羽佳,殷俏,郑琳璐,曾军.松果菊苷对高糖诱导的人视网膜毛细血管内皮细胞增殖、迁移及新生血管形成的影响[J].眼科新进展,2022,0(4):272-277. |
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| 作者姓名： | 毛羽佳 殷俏 郑琳璐 曾军 |
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| 作者单位： | 334000 江西省上饶市，江西医学高等专科学校第一附属医院眼科（毛羽佳，殷俏，郑琳璐）；410012 湖南省长沙市，中南大学湘雅二医院眼科（曾军） |
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| 摘 要： | 目的 探讨松果菊苷(ECH)对高糖诱导的人视网膜毛细血管内皮细胞(HRCECs)增殖、迁移及新生血管形成的影响及其作用机制.方法 将HRCECs分为5组:正常对照(Con)组、高糖组(HG,25 mmol·L-1 D-葡萄糖)、HG+1μmol·L-1 ECH组、HG+5μmol·L-1 ECH组和HG+25μmol·...
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| 关 键 词： | 松果菊苷 糖尿病视网膜病变 视网膜毛细血管内皮细胞 增殖 迁移 血管新生 HIF-1α/VEGF信号通路 |
Effect of echinacoside on proliferation,migration and angiogenesis of human retinal capillary endothelial cells induced by high glucose |
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| MAO Yujia,YIN Qiao,ZHENG Linlu,ZENG Jun.Effect of echinacoside on proliferation,migration and angiogenesis of human retinal capillary endothelial cells induced by high glucose[J].Recent Advances in Ophthalmology,2022,0(4):272-277. |
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| Authors: | MAO Yujia YIN Qiao ZHENG Linlu ZENG Jun |
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| Affiliation: | 1.Department of Ophthalmology,the First Affiliated Hospital of Jiangxi Medical College,Shangrao 334000,Jiangxi Province,China2.Department of Ophthalmology,the Second Xiangya Hospital of Central South University,Changsha 410012,Hunan Province,China |
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| Abstract: | Objective To investigate the effect of echinacoside (ECH) on proliferation, migration and angiogenesis of human retinal capillary endothelial cells (HRCECs) induced by high glucose, and to analyze its related mechanism. Methods HRCECs were divided into five groups: normal control group (Con), high glucose group (HG, 25 mmol·L-1 D-glucose), HG+1 μmol·L-1 ECH group, HG+5 μmol·L-1 ECH group, and HG+25 μmol·L-1 ECH group. Angiogenesis of HRCECs in each group was measured by tube formation assay; viability of HRCECs in each group was measured by MTT assay; colony formation of HRCECs in each group was tested by colony formation assay; migration of HRCECs in each group was detected by Transwell assay; proliferation of HRCECs in each group was evaluated by Ki67 antigen (Ki67) immunofluorescence staining; the positive expression of intercellular adhesion molecule 1 (ICAM-1) of HRCECs in each group was observed by immunofluorescence staining; and the expression levels of hypoxia-inducible factor 1α (HIF-1α) and vascular endothelial growth factor (VEGF) in HRCECs in each group were detected by Western blot.Results Compared with the Con group, the angiogenesis, viability, colony formation, proportion of Ki67-positive cells, and migration of HRCECs, as well as the expression levels of ICAM-1, HIF-1α and VEGF in the HG group were significantly increased (all P<0.001). Compared with the HG group, the above indexes in the HG+1 μmol·L-1 ECH group, HG+5 μmol·L-1 ECH group and HG+25 μmol·L-1 ECH group were decreased with the increase of ECH dose (P<0.05, P<0.01, and P<0.001).Conclusion ECH can inhibit the proliferation, migration and angiogenesis of HRCECs induced by HG, which may be related to its inhibition on HIF-1α/VEGF signaling pathway. |
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| Keywords: | echinacoside diabetic retinopathy retinal capillary endothelial cells proliferation migration angiogenesis hypoxia-inducible factor 1α/vascular endothelial growth factor signaling pathway |
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