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| 中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究 | |
| 引用本文： | 程春林,冯毅,何翔,林鹏,梁淑家,易志强,贺健梅,胡园园,邢辉,范雁,吴士良,邵一鸣.中国南方四省区流行的HIV-1 CRF01_AE病毒株基因特征研究[J].中华流行病学杂志,2009,30(7). |
| 作者姓名： | 程春林 冯毅 何翔 林鹏 梁淑家 易志强 贺健梅 胡园园 邢辉 范雁 吴士良 邵一鸣 |
| 作者单位： | 1. 中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制同家重点实验审,北京,100050 2. 广东省疾病预防控制国心 3. 广西壮族自治区疾病预防控制中心 4. 江西省疾病预防控制中心 5. 湖南省疾病预防控制中心 6. 中国疾病预防控制中心性病艾滋病预防控制中心传染病预防控制同家重点实验审,胡园园,北京,100050 7. 苏州大学医学部生物化学教研室 |
| 基金项目： | 国家高技术研究发展计划(863计划)，艾滋病和病毒性肝炎等重大传染病防治科技重大专项项目 |
| 摘 要： | 目的 分析中周南方四省区HIV-1感染者中流行CRF01 AE病毒株的遗传特征.方法 从广东、广西、江西和湖南省(自治区)2006年新报告HIV-1感染者的血浆样本中提取病毒RNA,用反转录/巢式PCR方法扩增gag和env基因片段,对获得的CRF01 AE病毒株核酸序列进行系统进化分析,并通过计算基因距离和Entropy核苷酸多态性差异方法分析毒株的遗传特征.结果 从210例CRF01_AE病毒株感染者中,发现四省区流行的CRF01 AE病毒株存在2个主要的流行簇.流行簇Ⅰ共有123例样本,未发现与之直接相关的国际参考毒株.流行簇Ⅱ共有57例样本,与越南CRF01 AE病毒株关系密切,且存在不同时间样本的混杂.gag和env基因遗传距离分析结果表明,流行簇Ⅰ内基因遗传多样性均明显小于流行簇Ⅱ;核苷酸多态性分析结果显示,在gag基因片段42个位点核苷酸组成具有显著差异,env基因片段40个位点核苷酸组成存在显著差异;流行簇Ⅰ相对于流行簇Ⅱ多态性减少的位点上有61个,多态性增加的位点有21个.结论 在中国南方四省区流行的CRF01 AE病毒株中首次观察到2个独立的流行簇.流行簇Ⅰ病毒株为该地区最主要的CRF01 AE病毒株,其流行时间相对较短,在人群中所占比例较多,可能是病毒在流行过程中形成的具有传播优势的病毒株.流行簇Ⅱ病毒与来自于越南的CRF01 AE病毒株有较高同源性,且存在与越南病毒株间的多次传播. |
| 关 键 词： | 病毒株 HIV-1 流行簇 进化分析 |
Genetic characteristics of HIV-1 CRF01-AE strains in four provinces, southern China |
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| CHENG Chun-lin,FENG Yi,HE Xiang,LIN Peng,LIANG Shu-jia,YI Zhi-qiang,HE Jian-mei,HU Yuan-yuan,XING Hui,FAN Yan,WU Shi-liang,SHAO Yi-ming.Genetic characteristics of HIV-1 CRF01-AE strains in four provinces, southern China[J].Chinese Journal of Epidemiology,2009,30(7). | |
| Authors: | CHENG Chun-lin FENG Yi HE Xiang LIN Peng LIANG Shu-jia YI Zhi-qiang HE Jian-mei HU Yuan-yuan XING Hui FAN Yan WU Shi-liang SHAO Yi-ming |
| Abstract: | Objective To analyze the genetic characteristics of HIV-1 CRF01_AE strains prevailing in. the four provinces of southern China. Methods Plasma samples were collected from the newly diagnosed HIV-1 individuals reported in 2006 in Guangdong, Guangxi, Jiangxi and Hunan province. The gag and env gene fragments were amplified from RNA template extracted from plasma using RT and nested PCR methods. CRF01_AE sequences were analyzed by phylogcnetie methods and characterized by calculating the genetic distance and Entropy analysis. Results Two main epidemic clusters were found to exist in the CRF01 AE strains from 210 HIV-1 CRF01 AE infected individuals collected in the 4 provinces, southern China. It was found that no international reference strain was closely correlated with cluster Ⅰ , which including 123 samples. The strains in cluster Ⅱ, consisting 57 cases of samples, were closely related with the strains identified in Vietnam. Genetic distance analysis of gag and env genes showed that the diversity of cluster Ⅰ was obviously less than that of cluster Ⅱ. Data on nucleotide polymorphism showed that nucleotides compositions of 42 sites in gag and 40 sites in env wer esignificantly different between the two clusters. When compared with cluster Ⅱ , the polymorphism decreased at 61 nucleotide sites but increased at 21 sites in cluster Ⅰ. Conclusion This was the first report describing that two main epidemic clusters were existed in CRF01_AE strains prevailing in the 4 provinces, Southern China. The vires in cluster Ⅰ was the dominant strain in this region, with shorter period of circulation and higher proportion seen in the HIV-infected population, which might belong to CRF01_AE strain with certain features facilitating the spread of the virus. The virus in cluster Ⅱ was highly homology with the CRF01_AE strains from Vietnam, and seemed to have had several events of epidemics in populations in border regions of China and Vietnam. |
| Keywords: | CRF01-AE |
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