| 姜黄素对氯化铝染毒的NG108 - 15细胞凋亡及PKC - NMDAR通路表达的影响 |
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| 引用本文： | 张露,陈媛,李俊良,张华,谢春.姜黄素对氯化铝染毒的NG108 - 15细胞凋亡及PKC - NMDAR通路表达的影响[J].现代预防医学,2022,0(16):3019-3024. |
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| 作者姓名： | 张露 陈媛 李俊良 张华 谢春 |
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| 作者单位： | 1．贵州医科大学公共卫生与健康学院，环境污染与疾病监控教育部重点实验室，贵州 贵阳 550025；2．贵州护理职业技术学院，基础医学部健康管理教研室，贵州 贵阳 550025 |
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| 摘 要： | 目的 探讨姜黄素对氯化铝染毒所致NG108 - 15细胞凋亡的影响及其机制，为铝致学习记忆损伤的治疗提供参考。方法 取对数生长期NG108 - 15细胞，随机分为空白对照组、DMSO组（溶剂对照）、姜黄素组（16 μmol/L姜黄素）、铝组（160 mg/L氯化铝染毒 ）、铝+姜黄素组（160 mg/L氯化铝染毒24 h后，给予16 μmol/L姜黄素处理24 h）。收集各组细胞，采用吖啶橙/嗅化乙锭（AO/EB）双荧光染色观察细胞凋亡形态，计数凋亡细胞数并计算凋亡率；流式细胞术检测细胞凋亡率， qRT - PCR检测细胞中PKC、NMDAR1、NMDAR2B 的mRNA表达水平，western blot检测细胞中凋亡相关蛋白（caspase3、Bax）和PKC、NMDAR1、NMDAR2B的蛋白表达水平。多组间均数的比较采用单因素方差分析（one - way ANOVA），组间两样本均数的比较采用LSD法。结果 与空白对照组相比，铝染毒组的caspase3、Bax蛋白表达水平升高（t = - 5.547、 - 4.948，P＜0.001），PKC、NMDAR1、NMDAR2B的mRNA（t = 4.926，P = 0.003；t = 6.330，P＜0.001；t = 4.224，P = 0.019）和蛋白（t = 20.638，P＜0.001；t = 4.509，P＜0.001；t = 17.388，P = 0.002）表达水平降低， AO/EB染色和流式细胞术结果均表明细胞凋亡率升高（t = - 5.153、 - 7.390，P＜0.001）；加入姜黄素处理后，与铝染毒组相比，铝+姜黄素组的caspase3、Bax蛋白表达水平降低（t = 2.930，P = 0.006；t = 4.907，P＜0.001），PKC、NMDAR1、NMDAR2B 的mRNA（t = - 10.337、 - 6.621、 - 6.847，P＜0.001）和蛋白（t = - 30.551、 - 7.451、 - 26.294，P＜0.001）表达水平升高，AO/EB染色和流式细胞术结果均表明细胞凋亡率降低（t = 2.707，P = 0.01；t = 4.632，P＜0.001）。结论 上调PKC - NMDAR信号通路表达，可能是姜黄素减轻氯化铝染毒所致NG108 - 15细胞凋亡的机制之一。
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| 关 键 词： | 铝 姜黄素 凋亡 PKC NMDAR |
Effect of curcumin on apoptosis and expression of PKC-NMDAR pathway of NG108-15 cells exposed to aluminum chloride |
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| ZHANG Lu,CHEN Yuan,LI Jun-liang,ZHANG Hua,XIE Chun.Effect of curcumin on apoptosis and expression of PKC-NMDAR pathway of NG108-15 cells exposed to aluminum chloride[J].Modern Preventive Medicine,2022,0(16):3019-3024. |
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| Authors: | ZHANG Lu CHEN Yuan LI Jun-liang ZHANG Hua XIE Chun |
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| Affiliation: | *School of Public Health, the Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education, Guizhou Medical University, Guiyang, Guizhou 550025, China |
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| Abstract: | Objective To investigate the effect of curcumin on the apoptosis of NG108-15 cells induced by aluminum chloride and its mechanism, and to provide reference for the treatment of aluminum induced learning and memory impairment. Methods NG108-15 cells at logarithmic growth stage were randomly divided into blank control group, DMSO group (solvent control), curcumin group (16 μmol/L curcumin), aluminum group (160 mg/L aluminum chloride) and aluminum + curcumin group (treated with 16 μmol/L curcumin after being exposed to 160 mg/L aluminum chloride for 24 h). Cells in each group were collected, the apoptotic morphology was observed by acridine orange/ethidium bromide (AO/EB) double fluorescence staining, and the number of apoptotic cells was counted and the apoptotic rate was calculated. Cell apoptosis rate was detected by flow cytometry, mRNA expression levels of PKC, NMDAR1 and NMDAR2B were detected by qRT-PCR, and apoptosis-related proteins (caspase3 and Bax) and protein expression levels of PKC, NMDAR1 and NMDAR2B were detected by western blot. One-way ANOVA was used for comparison of mean of multiple samples, and LSD was used for compare the mean of two samples between groups. Results Compared with the blank control group, the protein expression levels of caspase 3 and Bax increased (t=-5.547 and -4.948, P<0.001), the mRNA (t=4.926, P=0.003; t=6.330, P<0.001; t=4.224, P=(t=20.638, P<0.001; t=4.509, P<0.001; t=17.388, P=0.002) expression levels of PKC, NMDAR1 and NMDAR2B decreased, and the results of AO/EB staining and flow cytometry showed that the apoptosis rate was increased (t=-5.153 and -7.390, P<0.001). After treated with curcumin, the expression protein levels of caspase 3 and Bax in the aluminum + curcumin group decreased compared with the aluminum exposed group (t=2.930, P=0.006; t=4.907, P<0.001), and the mRNA (t=-10.337, -6.621, and -6.847, P<0.001) and protein (t=-30.551, -7.451, and -26.294, P<0.001) expression levels of PKC, NMDAR1 and NMDAR2B increased. The results of AO / EB staining and flow cytometry showed that the apoptosis rate decreased (t=2.707, P=0.01; t=4.632, P<0.001). Conclusion The up-regulated expression of PKC-NMDAR signaling pathway may be one of the mechanisms by which curcumin alleviates the apoptosis of NG108-15 cells induced by aluminum chloride exposure. |
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| Keywords: | Aluminum Curcumin Apoptosis PKC NMDAR |
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