| 2018—2021年北京市沙门菌血清型及喹诺酮类耐药表型和基因型分析 |
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| 引用本文： | 曲梅,田祎,黄瑛,张新,贾蕾,吕冰,张代涛.2018—2021年北京市沙门菌血清型及喹诺酮类耐药表型和基因型分析[J].现代预防医学,2022,0(13):2413-2419. |
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| 作者姓名： | 曲梅 田祎 黄瑛 张新 贾蕾 吕冰 张代涛 |
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| 作者单位： | 北京市疾病预防控制中心，食物中毒诊断溯源技术北京市重点实验室，北京 100013 |
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| 摘 要： | 目的 分析2018—2021年北京市肠道门诊哨点医院分离到的沙门菌血清型及喹诺酮类药物耐药表型和耐药基因的流行情况。方法 采用玻片凝集法鉴定血清型；微量肉汤稀释法测定药物敏感性；全基因组测序数据通过与MLST和ResFinder数据库比对，查询ST型别和耐药基因。结果 228株沙门菌共检出42种血清型，51种ST型。其中肯塔基沙门菌、肠炎沙门菌和黄金海岸沙门菌等9种血清型比较常见。77株沙门菌（33.8%）对萘啶酸耐药；40株（17.5%）对环丙沙星和左氧氟沙星耐药；38株（16.7%）对吉米沙星耐药。不同血清型对喹诺酮类药物的耐药性不同。228株沙门菌共筛选出5种喹诺酮耐药基因:qnrS（23.7%）、aac(6’)-Ib-cr（8.3%）、qnrB（6.6%）、OqxAB（1.3%）和qepA（0.4%）；喹诺酮耐药决定区存在gyrA和parC的7种氨基酸突变:以parC:T57S（70.2%）最常见，其次是gyrA:D87N（14.9%）、parC:S80I（14.5%）、gyrA:S83F（14.5%）、gyrA:D87Y（7.9%）、gyrA:D87G（5.3%）和gyrA:S83Y（1.8%）。在组合突变中，以parC:S80I-gyrA:D87N-gyrA:S83F三突变，并伴随parC:T57S最多。耐药基因和突变位点在9种常见血清型中的检出率存在差异。结论 北京市沙门菌血清型及ST型种类繁多，携带喹诺酮耐药基因以qnrS、aac(6’)-Ib-cr和 qnrB为主，存在gyrA和parC多个位点突变并协同作用。应持续监测血清型和耐药性，以指导临床有针对性合理用药。
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| 关 键 词： | 沙门菌 血清型 喹诺酮类抗生素 耐药基因 全基因组测序 |
Serotypes and quinolone resistant phenotypes and genotypes of Salmonella strains in Beijing, 2018—2021 |
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| QU Mei,TIAN Yi,HUANG Ying,ZHANG Xin,JIA Lei,LV Bing,ZHANG Dai-tao.Serotypes and quinolone resistant phenotypes and genotypes of Salmonella strains in Beijing, 2018—2021[J].Modern Preventive Medicine,2022,0(13):2413-2419. |
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| Authors: | QU Mei TIAN Yi HUANG Ying ZHANG Xin JIA Lei LV Bing ZHANG Dai-tao |
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| Affiliation: | Beijing Key Laboratory of Diagnostic and Traceability Technologies for Food Poisoning, Beijing Center for Disease Prevention and Control, Beijing 100013, China |
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| Abstract: | Objective To investigate the prevalence of serotyping, drug resistance and resistant genes associated with quinolone of Salmonella strains isolated from diarrheal outpatients in Beijing during 2018-2021. Methods The serovars of Salmonella strains were determined using slide agglutination method. The drug resistance was tested by using micro broth dilution method. STs and resistance genes were identified by comparing with MLST and ResFinder database using WGS data. Results Forty-two serotypes and 51 STs were identified among 228 Salmonella isolates. Nine kinds of serotypes were more common, including Salmonella Kentucky, S. Enteritidis and S. Goldcoast, etc. Of 228 Salmonella strains, 77 (33.8%) were resistant to nalidixic acid, 40 (17.5%) were resistant to ciprofloxacin and levofloxacin, and 38(16.7%) were resistant to gemifloxacin. The drug resistances of different serotypes to quinolones were significantly different. A total of 5 kinds of resistance genes associated to quinolones were screened. qnrS (23.7%) accounted for the most, and the detection rates of aac(6’)-Ib-cr, qnrB, OqxAB and qepA were 8.3%, 6.6%, 1.3% and 0.4%, respectively. Seven mutations of gyrA and parC in quinolone resistance-determining region (QRDR) were identified. The most frequent mutation was parC:T57S (70.2%), followed by gyrA:D87N (14.9%), parC:S80I (14.5%), gyrA:S83F (14.5%), gyrA:D87Y (7.9%), gyrA:D87G (5.3%) and gyrA:S83Y (1.8%). Among the combined mutations, the three mutation parC:S80I-gyrA:D87N-gyrA:S83F accompanied by parC:T57S was dominant. There were differences in the detection rates of resistant genes and mutations in the nine most common serotypes. Conclusion The serotypes and STs of Salmonella clinical strains in Beijing show a great variety. The quinolone resistant genes are mainly qnrS, aac(6’)-Ib-cr and qnrB, with synergistic effects in multiple mutations of gyrA and parC. It is necessary to continuously monitor serotype and drug resistance to guide targeted and rational drug use. |
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| Keywords: | Salmonella Serotyping Quinolones Resistance genes Whole-genome sequencing |
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