| 电感耦合等离子体-质谱法测定人尿中21种微量元素 |
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| 引用本文： | 王丽,朱军,吴倩,曹淑媛,李磊,程洁.电感耦合等离子体-质谱法测定人尿中21种微量元素[J].现代预防医学,2020,0(3):484-487. |
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| 作者姓名： | 王丽 朱军 吴倩 曹淑媛 李磊 程洁 |
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| 作者单位： | 1.南京医科大学公共卫生学院科研共享平台，江苏 南京 211166；2.南京医科大学公共卫生学院卫生检验学系， 江苏 南京 211166 |
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| 摘 要： | 目的 建立人尿中21种微量元素同时分析的电感耦合等离子体-质谱测定方法。方法 尿液经2%硝酸稀释10倍后，采用电感耦合等离子体（ICP）-四级杆质谱法（MS）检测。结果 采用内标法定量，各元素内标回归方程的决定系数均大于0.99，加标回收率在63.6%~101.5%之间。所测元素的相对标准偏差小于15%。各元素检出限在0.001μg/L~0.880μg/L之间。尿样内标元素45Sc的信号漂移在40%以内；74Ge，89Y和209Bi的信号漂移在30%以内；115In的信号漂移在20%以内。结论 ICP-MS方法简便、快速、灵敏、稳定，适合批量人尿中21种元素的分析。
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| 关 键 词： | 尿液 电感耦合-等离子体质谱法 微量元素 |
Determination of 21 trace elements in human urine by inductively coupled plasma-mass spectrometry |
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| WANG Li,ZHU Jun,WU Qian,CAO Shu-yuan,LI Lei,CHENG Jie.Determination of 21 trace elements in human urine by inductively coupled plasma-mass spectrometry[J].Modern Preventive Medicine,2020,0(3):484-487. |
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| Authors: | WANG Li ZHU Jun WU Qian CAO Shu-yuan LI Lei CHENG Jie |
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| Affiliation: | *Department of Core Facilities, School of Public Health, Nanjing Medical University, Nanjing, Jiangsu 211166, China |
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| Abstract: | Objective To establish a method for simultaneous analysis of 21 trace elements in human urine by inductively coupled plasma-mass spectrometry. Methods The urine was diluted with 2% nitric acid for 10 times, and detected by inductively coupled plasma-mass spectrometry(ICP-MS). Results Using the internal standard method, the determination coefficients of each element were all greater than 0.99, and the recoveries were between 63.6%～ 101.5%. The relative standard deviations(RSDs) were less than 15%. The detection limits were between 0.001 μg/L and 0.880 μg/L. The signal drift of 45 Sc in the urine sample was within 40 % and the signal drift of 74 Ge, 89 Y and 209 Bi was within 30%. The signal drift of 115 In was within 20%. Conclusion This method is simple, quick, sensitive and stable. Hence, it is suitable for the analysis of 21 trace elements in batch human urine. |
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| Keywords: | Urine Inductively coupled plasma-mass spectrometry Trace elements |
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