| 应用HIV - 1 DNA定量技术对集合核酸筛查法的改良研究 |
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| 引用本文： | 钟一帆,刘丹,甘永霞,黎桂连,高石兰,王晓辉,杨峥嵘.应用HIV - 1 DNA定量技术对集合核酸筛查法的改良研究[J].现代预防医学,2020,0(6):1087-1090. |
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| 作者姓名： | 钟一帆 刘丹 甘永霞 黎桂连 高石兰 王晓辉 杨峥嵘 |
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| 作者单位： | 1. 深圳市疾病预防控制中心艾滋病防制所，广东 深圳518055;2. 南华大学公共卫生学院，湖南 衡阳 421000 |
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| 摘 要： | 目的 研究HIV - 1 DNA定量检测用于改良集合核酸筛查的可行性。方法 建立荧光定量PCR检测HIV - 1 DNA的检测技术方法，选取来自深圳市各社区咨询检测点新报告HIV - 1抗体阳性者和阴性者血样，从检测样本量、保存条件等方面进行分组研究，寻找确保检出率的基本条件。在此基础上，结合病毒核酸浓缩，确定改良集合检测方案，并对自制的阴性、阳性外部质控品进行检测分析。结果 超敏HIV - 1 DNA检测技术在应用于不同病毒载量样本时，全血单次用量200 μl/反应实现了全部检出，而100 μl以下单次用量在低载量组血样中开始出现少量漏检；血样在室温3 d、4℃ 7 d、 - 20℃ 4周以内，以及 - 20℃保存冻融3次以内，均未对超敏HIV - 1 DNA定性/定量结果产生明显影响；本实验中通过改良集合核酸检测方案，对所有阳性质控品的检出率达100.0%，而对照组的一级集合样本检出率仅为86.3%（69/80）。结论 经过病毒核酸浓缩及超敏HIV - 1 DNA检测改良后的集合核酸检测方案可用于人群筛查。
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| 关 键 词： | 人类免疫缺陷病毒 艾滋病 核酸检测 |
Study of improved pooled nucleic acid amplification tests with nuclei acid quantification technology |
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| ZHONG Yi-fan,LIU Dan,GAN Yong-xia,LI Gui-lian,GAO Shi-lan,WANG Xiao-hui,YANG Zheng-rong.Study of improved pooled nucleic acid amplification tests with nuclei acid quantification technology[J].Modern Preventive Medicine,2020,0(6):1087-1090. |
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| Authors: | ZHONG Yi-fan LIU Dan GAN Yong-xia LI Gui-lian GAO Shi-lan WANG Xiao-hui YANG Zheng-rong |
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| Affiliation: | *Department of HIV/AIDS prevention and control, Shenzhen Center for Disease Control and prevention, Shenzhen Guangdong, 518055, China |
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| Abstract: | The aim of this study was to research the possibility of HIV detection with ultra nuclei acid quantification technology in early stage screening through pooled nucleic acid amplification testing(NAAT). Methods HIV-1 DNA detection with SurperBio ultra nuclei acid technology was established. The HIV positive and negative samples of new report were selected from local VCT, and the basic conditions was recorded to ensure the detection rate through detecting suitable volume, storage condition, etc. Based on the above research, standard pooling PCR plan were modified with virus concentration, and practiced in external quality control products. Results All HIV-1 positive samples were reported when samples usage was 200 ul/test. Missed detection began to appear when samples usage was less than 100 ul/test, especially in the low viral lord group. When samples were stored under room temperature for 3 days, 4℃ for 7 days,-20℃ within 4 weeks,and-20℃ freeze-thaw 3 times, no significant effect was observed for the results of SurperBio ultra nuclei acid test. Modified pooling PCR plan found 100% positive quality control products,while control group only found 86.3%(69/80). Conclusion Modified pooling PCR based on SurperBio ultra nuclei acid technology and virus concentration had potential in HIV mass screening. |
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| Keywords: | Human immunodeficiency virus Acquired immune deficiency syndrome Nucleic acid test |
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