| 我国柯萨奇病毒A组6型VP1区基因分子流行病学特征 |
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| 引用本文： | 范云燕,欧嵩凤,陈敏玫.我国柯萨奇病毒A组6型VP1区基因分子流行病学特征[J].中华疾病控制杂志,2020,24(8):934-938,945. |
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| 作者姓名： | 范云燕 欧嵩凤 陈敏玫 |
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| 作者单位： | 1.530023 南宁, 南宁市疾病预防控制中心 |
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| 摘 要： | 目的 分析我国2010―2018年柯萨奇病毒A组6型（Coxsackievirus A6，CV-A6）毒株VP1区基因流行和进化规律，为手足口病的防治提供科学依据。 方法 从GenBank获得2010―2018年我国CV-A6病毒全长VP1区核苷酸序列，用MEGA V7.0和Interactive Tree of Life V5（iTOLV5）软件构建进化树，用DNAstar V8.1.3软件对核苷酸同源性进行分析。 结果 880条序列来源的CV-A6毒株以D3亚型为主，占毒株总数的95.45%，不同基因型核苷酸同源性为80.8%~86.0%，同基因型内核苷酸同源性为88.1%~100.0%。D2亚型VP1区相对稳定，无整体氨基酸变异情况；而D3毒株VP1区发生多点氨基酸替换，其中A5T、T283A、N137S、V242I、A30V、I174V氨基酸替换循环出现，这种进化模式与越南地区相似，但与日本地区却不同。VP1区5T、30A、137N、242V比例逐年增加，可能与CV-A6引起我国手足口的轻症和重症病例比例上升有关。 结论 具有VP1区遗传多样性的CV-A6 D3株的出现可能是CV-A6在反复流行的一个重要因素，这有助于解释我国CV-A6的流行情况和流行特点。
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| 关 键 词： | 柯萨奇病毒A组6型 VP1区基因 分子流行病学 |
| 收稿时间： | 2020-02-01 |
Molecular epidemiological characterization for VP1 region gene of coxsackievirus A6 in China |
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| Affiliation: | 1.Nanning Center for Disease Control and Prevention, Nanning 530023, China2.Guangxi Center for Disease Control and Prevention, Nanning 530028, China |
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| Abstract: | Objective To analyze the epidemic and genetic evolution principals of Coxsackievirus group A type 6 (CV-A6) VP1 region from 2010 to 2018 in China so as to provide scientific basis for the prevention and treatment of Hand-foot-mouth disease (HFMD). Methods The full-length VP1 nucleotide sequence of CV-A6 virus from 2010 to 2018 in China was obtained from GenBank. The phylogenetic trees based on VP1 sequences of CV-A6 were constructed by the MEGA V7.0 and Interactive Tree of Life V5 (iTOLV5) software, and the nucleotide homology was analyzed with DNAstar V8.1.3. Results The CV-A6 D3 was the main sub-genotype, accounting for 95.45% of the total 880 strains. The homology of nucleotides sequences in different genotype was between 80.8% and 86.0% while the homology of nucleotides sequences in the same genotype was between 88.1% and 100.0%.The VP1 sequences of D2 was relatively stable without any global amino acid variation. However, the VP1 sequences of D3 had multi-point amino acid substitutions, in which A5T, T283A, N137S, V242I, A30V and I174V amino acid substitution occurred circularly. This evolutionary pattern was similar to that in Vietnam but different from that in Japan. The proportion of 5T, 30A, 137N and 242V in the VP1 region increased year by year, which may be related to the increasing proportion of the mild and severe cases of HFMD caused by CV-A6 in China. Conclusions The emergence of CV-A6 D3 Sub-genotype strains with high genetic diversity in VP1 region could be a factor associated with the repeated epidemics, which helps to explain the epidemics and characteristics of CV-A6 in China. |
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