| 中国柯萨奇病毒A组2型的遗传进化分析 |
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| 引用本文： | 梁若楠,孙甜甜,纪望全,陈帅印,杨海燕,段广才,晋乐飞.中国柯萨奇病毒A组2型的遗传进化分析[J].中华疾病控制杂志,2022,26(8):982-988. |
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| 作者姓名： | 梁若楠 孙甜甜 纪望全 陈帅印 杨海燕 段广才 晋乐飞 |
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| 作者单位： | 450001 郑州，郑州大学公共卫生学院流行病学教研室 |
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| 基金项目： | 国家自然科学基金82002147国家自然科学基金82073618中国博士后科学基金2019M662543河南省高等学校重点科研项目20A330004河南省高等学校重点科研项目21A310026 |
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| 摘 要： | 目的 探讨中国柯萨奇病毒A组2型(coxsackievirus A2, CV-A2)毒株的遗传进化特征。 方法 收集中国CV-A2毒株的VP1区及全基因序列，采用MEGA 10.2.4软件构建系统进化树并开展氨基酸位点突变分析，采用SimPlot 3.5.1软件进行基因重组分析。 结果 共收集到118条VP1序列和26条全基因序列。CV-A2毒株主要来源于广东省，毒株数量自2011年开始迅速增多。基于VP1序列构建的进化树有7个分支，原型毒株为独立A组，多数CV-A2毒株聚集于G组。具有全基因组序列的CV-A2代表株的基因重组分析显示毒株间存在基因重组。另外，VP1氨基酸序列共有22个突变位点。 结论 中国流行的CV-A2毒株已发生较明显的基因重组和VP1区域氨基酸位点突变，监测CV-A2的演化对于手足口病的防控具有重要意义。
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| 关 键 词： | 柯萨奇病毒A组2型 系统进化 基因重组 |
| 收稿时间： | 2021-12-03 |
Phylogenetic analysis of coxsackievirus A2 in China |
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| Affiliation: | Department of Epidemiology, School of Public Health, Zhengzhou University, Zhengzhou 450001, China |
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| Abstract: | Objective To analyze the phylogenetic character of coxsackievirus A2 (CV-A2) strains in China. Methods Sequences of the VP1 region and complete genomes of CV-A2 strains in China were collected. The phylogenetic tree and analysis of amino acid mutation were conducted by MEGA 10.2.4 software. The analysis of genetic recombination was carried out through SimPlot 3.5.1 software. Results A total of 118 sequences of CV-A2 VP1 were collected, and 26 strains had complete genome sequences. CV-A2 strains mainly originated from Guangdong Province. The number of CV-A2 strains had increased rapidly since 2011. Phylogenetic tree based on the VP1 region was clustered into seven distinct lineages, and the prototype strains were a separate lineage A while most of strains gathered in lineages G. The analysis of gene recombination of CV-A2 representative strains with complete genome sequences exhibited recombinant segments. The amino acid variations were found at 22 sites of VP1 protein. Conclusion CV-A2 strains circulating in China have undergone gene recombination and mutations in the amino acid sites of the VP1 region, suggesting that monitoring the evolution of CV-A2 is of great significance for the prevention and control of hand-foot-mouth disease. |
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